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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Danio rerio Histone H2A.V (h2afv) CSB-YP747884DIL
CSB-EP747884DIL
CSB-BP747884DIL
CSB-MP747884DIL
CSB-EP747884DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease PA CSB-YP747885VDN
CSB-EP747885VDN
CSB-BP747885VDN
CSB-MP747885VDN
CSB-EP747885VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease KN9 CSB-YP747886VDN
CSB-EP747886VDN
CSB-BP747886VDN
CSB-MP747886VDN
CSB-EP747886VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease KN4 homolog CSB-YP747887VDN
CSB-EP747887VDN
CSB-BP747887VDN
CSB-MP747887VDN
CSB-EP747887VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane and ubiquitin-like domain-containing protein 2 (TMUB2), partial CSB-YP747888HU1
CSB-EP747888HU1
CSB-BP747888HU1
CSB-MP747888HU1
CSB-EP747888HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Kynurenine--oxoglutarate transaminase 3 (Ccbl2) CSB-YP747889MO
CSB-EP747889MO
CSB-BP747889MO
CSB-MP747889MO
CSB-EP747889MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Stejaggregin-B subunit beta-2 CSB-YP747890VDN
CSB-EP747890VDN
CSB-BP747890VDN
CSB-MP747890VDN
CSB-EP747890VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinonyx jubatus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP747891AFAO1
CSB-EP747891AFAO1
CSB-BP747891AFAO1
CSB-MP747891AFAO1
CSB-EP747891AFAO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Out at first protein homolog (oaf) CSB-YP747892CH
CSB-EP747892CH
CSB-BP747892CH
CSB-MP747892CH
CSB-EP747892CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sminthopsis virginiae Sperm protamine P1 (PRM1) CSB-YP747893SGAL
CSB-EP747893SGAL
CSB-BP747893SGAL
CSB-MP747893SGAL
CSB-EP747893SGAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Histone H2A.V (H2AFV) CSB-YP747894HU
CSB-BP747894HU
CSB-MP747894HU
CSB-EP747894HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phascogale calura Sperm protamine P1 (PRM1) CSB-YP747895PCAK
CSB-EP747895PCAK
CSB-BP747895PCAK
CSB-MP747895PCAK
CSB-EP747895PCAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antechinus minimus Sperm protamine P1 (PRM1) CSB-YP747896BZW
CSB-EP747896BZW
CSB-BP747896BZW
CSB-MP747896BZW
CSB-EP747896BZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sperm protamine P1 (PRM1) CSB-YP747897MWR
CSB-EP747897MWR
CSB-BP747897MWR
CSB-MP747897MWR
CSB-EP747897MWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ningaui timealeyi Sperm protamine P1 (PRM1) CSB-YP747898NHF
CSB-EP747898NHF
CSB-BP747898NHF
CSB-MP747898NHF
CSB-EP747898NHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b L-rhamnose mutarotase (rhaM) CSB-YP747899LAAD
CSB-EP747899LAAD
CSB-BP747899LAAD
CSB-MP747899LAAD
CSB-EP747899LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP747900LAAD
CSB-EP747900LAAD
CSB-BP747900LAAD
CSB-MP747900LAAD
CSB-EP747900LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Macro domain-containing protein LMOf2365_2748 (LMOf2365_2748) CSB-YP747901LAAD
CSB-EP747901LAAD
CSB-BP747901LAAD
CSB-MP747901LAAD
CSB-EP747901LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Recombination protein RecR (recR) CSB-YP747902LAAD
CSB-EP747902LAAD
CSB-BP747902LAAD
CSB-MP747902LAAD
CSB-EP747902LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Elongation factor G (fusA), partial CSB-YP747903LAAD
CSB-EP747903LAAD
CSB-BP747903LAAD
CSB-MP747903LAAD
CSB-EP747903LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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