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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus FMN-dependent NADPH-azoreductase (azo1) CSB-YP750046SKW
CSB-EP750046SKW
CSB-BP750046SKW
CSB-MP750046SKW
CSB-EP750046SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative pyridoxal phosphate-dependent acyltransferase (SAS0508) CSB-YP750047SKW
CSB-EP750047SKW
CSB-BP750047SKW
CSB-MP750047SKW
CSB-EP750047SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cysteine--tRNA ligase (cysS) CSB-YP750048SKW
CSB-EP750048SKW
CSB-BP750048SKW
CSB-MP750048SKW
CSB-EP750048SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Hypoxanthine-guanine phosphoribosyltransferase (hpt) CSB-YP750049SKW
CSB-EP750049SKW
CSB-BP750049SKW
CSB-MP750049SKW
CSB-EP750049SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L25 (rplY) CSB-YP750050SKW
CSB-EP750050SKW
CSB-BP750050SKW
CSB-MP750050SKW
CSB-EP750050SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0213 protein SAS0445 (SAS0445) CSB-YP750051SKW
CSB-EP750051SKW
CSB-BP750051SKW
CSB-MP750051SKW
CSB-EP750051SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP750052SKW
CSB-EP750052SKW
CSB-BP750052SKW
CSB-MP750052SKW
CSB-EP750052SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alkyl hydroperoxide reductase subunit C (ahpC) CSB-YP750053SKW
CSB-EP750053SKW
CSB-BP750053SKW
CSB-MP750053SKW
CSB-EP750053SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein EssC (essC), partial CSB-YP750054SKW
CSB-EP750054SKW
CSB-BP750054SKW
CSB-MP750054SKW
CSB-EP750054SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycyl-glycine endopeptidase lytM (lytM) CSB-YP750055SKW
CSB-EP750055SKW
CSB-BP750055SKW
CSB-MP750055SKW
CSB-EP750055SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Antiholin-like protein LrgA (lrgA), partial CSB-YP750056SKW1
CSB-EP750056SKW1
CSB-BP750056SKW1
CSB-MP750056SKW1
CSB-EP750056SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 (ispD1) CSB-YP750057SKW
CSB-EP750057SKW
CSB-BP750057SKW
CSB-MP750057SKW
CSB-EP750057SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus PTS system EIIBC component SAS0167 (SAS0167), partial CSB-YP750058SKW
CSB-EP750058SKW
CSB-BP750058SKW
CSB-MP750058SKW
CSB-EP750058SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP750059SKW
CSB-EP750059SKW
CSB-BP750059SKW
CSB-MP750059SKW
CSB-EP750059SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Superoxide dismutase [Mn/Fe] 2 (sodM) CSB-YP750060SKW
CSB-EP750060SKW
CSB-BP750060SKW
CSB-MP750060SKW
CSB-EP750060SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP750061SKW
CSB-EP750061SKW
CSB-BP750061SKW
CSB-MP750061SKW
CSB-EP750061SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L9 (rplI) CSB-YP750062SKW
CSB-EP750062SKW
CSB-BP750062SKW
CSB-MP750062SKW
CSB-EP750062SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA polymerase III subunit beta (dnaN) CSB-YP750063SKW
CSB-EP750063SKW
CSB-BP750063SKW
CSB-MP750063SKW
CSB-EP750063SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0397 protein SAR2767 (SAR2767), partial CSB-YP750064SKV1
CSB-EP750064SKV1
CSB-BP750064SKV1
CSB-MP750064SKV1
CSB-EP750064SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP750065SKV
CSB-EP750065SKV
CSB-BP750065SKV
CSB-MP750065SKV
CSB-EP750065SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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