Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio vulnificus UPF0231 protein VV2750 (VV2750) CSB-YP748824VCQ
CSB-EP748824VCQ
CSB-BP748824VCQ
CSB-MP748824VCQ
CSB-EP748824VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Translation initiation factor IF-2 (infB), partial CSB-YP748825VCQ
CSB-EP748825VCQ
CSB-BP748825VCQ
CSB-MP748825VCQ
CSB-EP748825VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphopentomutase (deoB) CSB-YP748826VCQ
CSB-EP748826VCQ
CSB-BP748826VCQ
CSB-MP748826VCQ
CSB-EP748826VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Na (+)-translocating NADH-quinone reductase subunit B (nqrB), partial CSB-YP748827VCQ
CSB-EP748827VCQ
CSB-BP748827VCQ
CSB-MP748827VCQ
CSB-EP748827VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Elongation factor Ts (tsf) CSB-YP748828VCQ
CSB-EP748828VCQ
CSB-BP748828VCQ
CSB-MP748828VCQ
CSB-EP748828VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP748829VCQ
CSB-EP748829VCQ
CSB-BP748829VCQ
CSB-MP748829VCQ
CSB-EP748829VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon (cheB1) CSB-YP748830VCQ
CSB-EP748830VCQ
CSB-BP748830VCQ
CSB-MP748830VCQ
CSB-EP748830VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Recombination protein RecR (recR) CSB-YP748831VCQ
CSB-EP748831VCQ
CSB-BP748831VCQ
CSB-MP748831VCQ
CSB-EP748831VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0434 protein VV2354 (VV2354) CSB-YP748832VCQ
CSB-EP748832VCQ
CSB-BP748832VCQ
CSB-MP748832VCQ
CSB-EP748832VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Translation initiation factor IF-1 (infA) CSB-YP748833VCQ
CSB-EP748833VCQ
CSB-BP748833VCQ
CSB-MP748833VCQ
CSB-EP748833VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus HTH-type transcriptional repressor PurR (purR) CSB-YP748834VCQ
CSB-EP748834VCQ
CSB-BP748834VCQ
CSB-MP748834VCQ
CSB-EP748834VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP748835VCQ
CSB-EP748835VCQ
CSB-BP748835VCQ
CSB-MP748835VCQ
CSB-EP748835VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Acetate kinase 1 (ackA1) CSB-YP748836VCQ
CSB-EP748836VCQ
CSB-BP748836VCQ
CSB-MP748836VCQ
CSB-EP748836VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Lipase chaperone (lifO), partial CSB-YP748837VCQ
CSB-EP748837VCQ
CSB-BP748837VCQ
CSB-MP748837VCQ
CSB-EP748837VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Multidrug resistance protein norM (norM), partial CSB-YP748838VCQ
CSB-EP748838VCQ
CSB-BP748838VCQ
CSB-MP748838VCQ
CSB-EP748838VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP748839VCQ
CSB-EP748839VCQ
CSB-BP748839VCQ
CSB-MP748839VCQ
CSB-EP748839VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Vitamin B12 import ATP-binding protein BtuD (btuD) CSB-YP748840VCQ
CSB-EP748840VCQ
CSB-BP748840VCQ
CSB-MP748840VCQ
CSB-EP748840VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphoserine aminotransferase (serC) CSB-YP748841VCQ
CSB-EP748841VCQ
CSB-BP748841VCQ
CSB-MP748841VCQ
CSB-EP748841VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Thymidine kinase (tdk) CSB-YP748842VCQ
CSB-EP748842VCQ
CSB-BP748842VCQ
CSB-MP748842VCQ
CSB-EP748842VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Imidazole glycerol phosphate synthase subunit hisF1 (hisF1) CSB-YP748843VCQ
CSB-EP748843VCQ
CSB-BP748843VCQ
CSB-MP748843VCQ
CSB-EP748843VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>