Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP750006SKW
CSB-EP750006SKW
CSB-BP750006SKW
CSB-MP750006SKW
CSB-EP750006SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aminoacyltransferase FemA (femA) CSB-YP750007SKW
CSB-EP750007SKW
CSB-BP750007SKW
CSB-MP750007SKW
CSB-EP750007SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphatidylglycerol lysyltransferase (mprF), partial CSB-YP750008SKW
CSB-EP750008SKW
CSB-BP750008SKW
CSB-MP750008SKW
CSB-EP750008SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Large-conductance mechanosensitive channel (mscL), partial CSB-YP750009SKW
CSB-EP750009SKW
CSB-BP750009SKW
CSB-MP750009SKW
CSB-EP750009SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Nuclease sbcCD subunit D (sbcD) CSB-YP750010SKW
CSB-EP750010SKW
CSB-BP750010SKW
CSB-MP750010SKW
CSB-EP750010SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamine synthetase (glnA) CSB-YP750011SKW
CSB-EP750011SKW
CSB-BP750011SKW
CSB-MP750011SKW
CSB-EP750011SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP750012SKW
CSB-EP750012SKW
CSB-BP750012SKW
CSB-MP750012SKW
CSB-EP750012SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Proline--tRNA ligase (proS), partial CSB-YP750013SKW
CSB-EP750013SKW
CSB-BP750013SKW
CSB-MP750013SKW
CSB-EP750013SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphatidate cytidylyltransferase (cdsA), partial CSB-YP750014SKW1
CSB-EP750014SKW1
CSB-BP750014SKW1
CSB-MP750014SKW1
CSB-EP750014SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor Ts (tsf) CSB-YP750015SKW
CSB-EP750015SKW
CSB-BP750015SKW
CSB-MP750015SKW
CSB-EP750015SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent protease subunit HslV (hslV) CSB-YP750016SKW
CSB-EP750016SKW
CSB-BP750016SKW
CSB-MP750016SKW
CSB-EP750016SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease HII (rnhB) CSB-YP750017SKW
CSB-EP750017SKW
CSB-BP750017SKW
CSB-MP750017SKW
CSB-EP750017SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease 3 (rnc) CSB-YP750018SKW
CSB-EP750018SKW
CSB-BP750018SKW
CSB-MP750018SKW
CSB-EP750018SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L28 (rpmB) CSB-YP750019SKW
CSB-EP750019SKW
CSB-BP750019SKW
CSB-MP750019SKW
CSB-EP750019SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Carbamoyl-phosphate synthase small chain (carA) CSB-YP750020SKW
CSB-EP750020SKW
CSB-BP750020SKW
CSB-MP750020SKW
CSB-EP750020SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lipoprotein signal peptidase (lspA), partial CSB-YP750021SKW1
CSB-EP750021SKW1
CSB-BP750021SKW1
CSB-MP750021SKW1
CSB-EP750021SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Non-canonical purine NTP pyrophosphatase (SAS1085) CSB-YP750022SKW
CSB-EP750022SKW
CSB-BP750022SKW
CSB-MP750022SKW
CSB-EP750022SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-regulated surface determinant protein C (isdC) CSB-YP750023SKW
CSB-EP750023SKW
CSB-BP750023SKW
CSB-MP750023SKW
CSB-EP750023SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heme A synthase (ctaA), partial CSB-YP750024SKW1
CSB-EP750024SKW1
CSB-BP750024SKW1
CSB-MP750024SKW1
CSB-EP750024SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyruvate dehydrogenase E1 component subunit beta (pdhB) CSB-YP750025SKW
CSB-EP750025SKW
CSB-BP750025SKW
CSB-MP750025SKW
CSB-EP750025SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>