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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rhodopirellula baltica tRNA (Ile)-lysidine synthase (tilS) CSB-YP749404RDR
CSB-EP749404RDR
CSB-BP749404RDR
CSB-MP749404RDR
CSB-EP749404RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Undecaprenyl-diphosphatase (uppP), partial CSB-YP749405RDR
CSB-EP749405RDR
CSB-BP749405RDR
CSB-MP749405RDR
CSB-EP749405RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica DNA mismatch repair protein MutS (mutS), partial CSB-YP749406RDR
CSB-EP749406RDR
CSB-BP749406RDR
CSB-MP749406RDR
CSB-EP749406RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Triosephosphate isomerase (tpiA) CSB-YP749407RDR
CSB-EP749407RDR
CSB-BP749407RDR
CSB-MP749407RDR
CSB-EP749407RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP749408RDR
CSB-EP749408RDR
CSB-BP749408RDR
CSB-MP749408RDR
CSB-EP749408RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Phosphoribosylamine--glycine ligase (purD) CSB-YP749409RDR
CSB-EP749409RDR
CSB-BP749409RDR
CSB-MP749409RDR
CSB-EP749409RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica UPF0365 protein RB6291 (RB6291), partial CSB-YP749410RDR1
CSB-EP749410RDR1
CSB-BP749410RDR1
CSB-MP749410RDR1
CSB-EP749410RDR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Acetylglutamate kinase (argB) CSB-YP749411RDR
CSB-EP749411RDR
CSB-BP749411RDR
CSB-MP749411RDR
CSB-EP749411RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica GTPase Der (der) CSB-YP749412RDR
CSB-EP749412RDR
CSB-BP749412RDR
CSB-MP749412RDR
CSB-EP749412RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Ribosome-binding factor A (rbfA) CSB-YP749413RDR
CSB-EP749413RDR
CSB-BP749413RDR
CSB-MP749413RDR
CSB-EP749413RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Thiazole synthase (thiG) CSB-YP749414RDR
CSB-EP749414RDR
CSB-BP749414RDR
CSB-MP749414RDR
CSB-EP749414RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica UPF0337 protein RB4474 (RB4474) CSB-YP749415RDR
CSB-EP749415RDR
CSB-BP749415RDR
CSB-MP749415RDR
CSB-EP749415RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Pantothenate synthetase (panC) CSB-YP749416RDR
CSB-EP749416RDR
CSB-BP749416RDR
CSB-MP749416RDR
CSB-EP749416RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Deoxycytidine triphosphate deaminase (dcd) CSB-YP749417RDR
CSB-EP749417RDR
CSB-BP749417RDR
CSB-MP749417RDR
CSB-EP749417RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP749418RDR
CSB-EP749418RDR
CSB-BP749418RDR
CSB-MP749418RDR
CSB-EP749418RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Glucosamine-6-phosphate deaminase (nagB) CSB-YP749419RDR
CSB-EP749419RDR
CSB-BP749419RDR
CSB-MP749419RDR
CSB-EP749419RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Queuine tRNA-ribosyltransferase (tgt) CSB-YP749420RDR
CSB-EP749420RDR
CSB-BP749420RDR
CSB-MP749420RDR
CSB-EP749420RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP749421RDR
CSB-EP749421RDR
CSB-BP749421RDR
CSB-MP749421RDR
CSB-EP749421RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica UPF0178 protein RB974 (RB974) CSB-YP749422RDR
CSB-EP749422RDR
CSB-BP749422RDR
CSB-MP749422RDR
CSB-EP749422RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Histidine--tRNA ligase (hisS) CSB-YP749423RDR
CSB-EP749423RDR
CSB-BP749423RDR
CSB-MP749423RDR
CSB-EP749423RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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