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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus subsp. pastoris Aspartate--tRNA ligase (aspS), partial CSB-YP749424EYQ
CSB-EP749424EYQ
CSB-BP749424EYQ
CSB-MP749424EYQ
CSB-EP749424EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP749425EYQ
CSB-EP749425EYQ
CSB-BP749425EYQ
CSB-MP749425EYQ
CSB-EP749425EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Protein translocase subunit SecA (secA), partial CSB-YP749426EYQ
CSB-EP749426EYQ
CSB-BP749426EYQ
CSB-MP749426EYQ
CSB-EP749426EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Photosystem I iron-sulfur center (psaC) CSB-YP749427EYQ
CSB-EP749427EYQ
CSB-BP749427EYQ
CSB-MP749427EYQ
CSB-EP749427EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris NAD (P)H-quinone oxidoreductase subunit N (ndhN) CSB-YP749428EYQ
CSB-EP749428EYQ
CSB-BP749428EYQ
CSB-MP749428EYQ
CSB-EP749428EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L16 (rplP) CSB-YP749429EYQ
CSB-EP749429EYQ
CSB-BP749429EYQ
CSB-MP749429EYQ
CSB-EP749429EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L15 (rplO) CSB-YP749430EYQ
CSB-EP749430EYQ
CSB-BP749430EYQ
CSB-MP749430EYQ
CSB-EP749430EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L31 (rpmE) CSB-YP749431EYQ
CSB-EP749431EYQ
CSB-BP749431EYQ
CSB-MP749431EYQ
CSB-EP749431EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Ribonuclease HII (rnhB) CSB-YP749432EYQ
CSB-EP749432EYQ
CSB-BP749432EYQ
CSB-MP749432EYQ
CSB-EP749432EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP749433EYQ
CSB-EP749433EYQ
CSB-BP749433EYQ
CSB-MP749433EYQ
CSB-EP749433EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP749435EYQ
CSB-EP749435EYQ
CSB-BP749435EYQ
CSB-MP749435EYQ
CSB-EP749435EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP749436EYQ
CSB-EP749436EYQ
CSB-BP749436EYQ
CSB-MP749436EYQ
CSB-EP749436EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris D-alanine--D-alanine ligase (ddl) CSB-YP749437EYQ
CSB-EP749437EYQ
CSB-BP749437EYQ
CSB-MP749437EYQ
CSB-EP749437EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Probable inorganic polyphosphate/ATP-NAD kinase 2 (ppnK2) CSB-YP749438EYQ
CSB-EP749438EYQ
CSB-BP749438EYQ
CSB-MP749438EYQ
CSB-EP749438EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP749439EYQ1
CSB-EP749439EYQ1
CSB-BP749439EYQ1
CSB-MP749439EYQ1
CSB-EP749439EYQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Biotin synthase (bioB) CSB-YP749440EYQ
CSB-EP749440EYQ
CSB-BP749440EYQ
CSB-MP749440EYQ
CSB-EP749440EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP749441EYQ
CSB-EP749441EYQ
CSB-BP749441EYQ
CSB-MP749441EYQ
CSB-EP749441EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Urease subunit alpha (ureC), partial CSB-YP749442EYQ
CSB-EP749442EYQ
CSB-BP749442EYQ
CSB-MP749442EYQ
CSB-EP749442EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L28 (rpmB) CSB-YP749443EYQ
CSB-EP749443EYQ
CSB-BP749443EYQ
CSB-MP749443EYQ
CSB-EP749443EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L32 (rpmF) CSB-YP749444EYQ
CSB-EP749444EYQ
CSB-BP749444EYQ
CSB-MP749444EYQ
CSB-EP749444EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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