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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Frog virus 3 Uncharacterized protein 074L (FV3-074L) CSB-YP750226FDG
CSB-EP750226FDG
CSB-BP750226FDG
CSB-MP750226FDG
CSB-EP750226FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Putative DNA-directed RNA polymerase II subunit RPB2 homolog (FV3-062L), partial CSB-YP750227FDG
CSB-EP750227FDG
CSB-BP750227FDG
CSB-MP750227FDG
CSB-EP750227FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Putative helicase 055L (FV3-055L) CSB-YP750228FDG
CSB-EP750228FDG
CSB-BP750228FDG
CSB-MP750228FDG
CSB-EP750228FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Uncharacterized protein 086L (FV3-086L) CSB-YP750229FDG
CSB-EP750229FDG
CSB-BP750229FDG
CSB-MP750229FDG
CSB-EP750229FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Uncharacterized protein 024R (FV3-024R) CSB-YP750230FDG
CSB-EP750230FDG
CSB-BP750230FDG
CSB-MP750230FDG
CSB-EP750230FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Uncharacterized protein 020R (FV3-020R), partial CSB-YP750231FDG
CSB-EP750231FDG
CSB-BP750231FDG
CSB-MP750231FDG
CSB-EP750231FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frog virus 3 Putative helicase 009L (FV3-009L), partial CSB-YP750232FDG
CSB-EP750232FDG
CSB-BP750232FDG
CSB-MP750232FDG
CSB-EP750232FDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon 50S ribosomal protein L16 (rplP) CSB-YP750233FCY
CSB-EP750233FCY
CSB-BP750233FCY
CSB-MP750233FCY
CSB-EP750233FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Retinoic acid early transcript 1G protein (RAET1G), partial CSB-YP750234HU
CSB-EP750234HU
CSB-BP750234HU
CSB-MP750234HU
CSB-EP750234HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable glucuronosyltransferase Os02g0520750 (Os02g0520750, LOC_Os02g32110), partial CSB-YP750235OFG
CSB-EP750235OFG
CSB-BP750235OFG
CSB-MP750235OFG
CSB-EP750235OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Expansin-B11 (EXPB11) CSB-YP750236OFG
CSB-EP750236OFG
CSB-BP750236OFG
CSB-MP750236OFG
CSB-EP750236OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Auxin response factor 6 (ARF6), partial CSB-YP750237OFG
CSB-EP750237OFG
CSB-BP750237OFG
CSB-MP750237OFG
CSB-EP750237OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative protein phosphatase 2C 23 (Os02g0633700, LOC_Os02g42250) CSB-YP750238OFG
CSB-EP750238OFG
CSB-BP750238OFG
CSB-MP750238OFG
CSB-EP750238OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Formin-like protein 10 (FH10), partial CSB-YP750239OFG
CSB-EP750239OFG
CSB-BP750239OFG
CSB-MP750239OFG
CSB-EP750239OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Type 2 lactosamine alpha-2,3-sialyltransferase (ST3GAL6), partial CSB-YP750240BO
CSB-EP750240BO
CSB-BP750240BO
CSB-MP750240BO
CSB-EP750240BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equisetum laevigatum 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP750241ECAT
CSB-EP750241ECAT
CSB-BP750241ECAT
CSB-MP750241ECAT
CSB-EP750241ECAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S18 (rpsR) CSB-YP750242BAAE
CSB-EP750242BAAE
CSB-BP750242BAAE
CSB-MP750242BAAE
CSB-EP750242BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian FMN-dependent NADH-azoreductase 3 (azoR3) CSB-YP750243BAAE
CSB-EP750243BAAE
CSB-BP750243BAAE
CSB-MP750243BAAE
CSB-EP750243BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0447 protein BT9727_5069 (BT9727_5069) CSB-YP750244BAAE
CSB-EP750244BAAE
CSB-BP750244BAAE
CSB-MP750244BAAE
CSB-EP750244BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP750245BAAE
CSB-EP750245BAAE
CSB-BP750245BAAE
CSB-MP750245BAAE
CSB-EP750245BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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