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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis Pancreatic progenitor cell differentiation and proliferation factor (ppdpf) CSB-YP003681XBF
CSB-EP003681XBF
CSB-BP003681XBF
CSB-MP003681XBF
CSB-EP003681XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein FAM132A (fam132a) CSB-YP008058XBF
CSB-EP008058XBF
CSB-BP008058XBF
CSB-MP008058XBF
CSB-EP008058XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Helicase ARIP4 (rad54l2), partial CSB-YP019272XBF
CSB-EP019272XBF
CSB-BP019272XBF
CSB-MP019272XBF
CSB-EP019272XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Large neutral amino acids transporter small subunit 4 (slc43a2), partial CSB-YP021646XBF
CSB-EP021646XBF
CSB-BP021646XBF
CSB-MP021646XBF
CSB-EP021646XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Tetratricopeptide repeat protein 30A (ttc30a), partial CSB-YP025224XBF
CSB-EP025224XBF
CSB-BP025224XBF
CSB-MP025224XBF
CSB-EP025224XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Tetratricopeptide repeat protein 9C (ttc9c) CSB-YP025247XBF
CSB-EP025247XBF
CSB-BP025247XBF
CSB-MP025247XBF
CSB-EP025247XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Oxidoreductase NAD-binding domain-containing protein 1 (oxnad1) CSB-YP017311XBF
CSB-EP017311XBF
CSB-BP017311XBF
CSB-MP017311XBF
CSB-EP017311XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Eukaryotic translation initiation factor 3 subunit A (eif3a), partial CSB-YP007543XBF
CSB-EP007543XBF
CSB-BP007543XBF
CSB-MP007543XBF
CSB-EP007543XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis N-acetylaspartate synthetase (nat8l), partial CSB-YP015476XBF1
CSB-EP015476XBF1
CSB-BP015476XBF1
CSB-MP015476XBF1
CSB-EP015476XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Insulin-like growth factor-binding protein 2 (igfbp2) CSB-YP011096XBF
CSB-EP011096XBF
CSB-BP011096XBF
CSB-MP011096XBF
CSB-EP011096XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein SDA1 homolog (sdad1), partial CSB-YP020885XBF
CSB-EP020885XBF
CSB-BP020885XBF
CSB-MP020885XBF
CSB-EP020885XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Solute carrier family 26 member 9 (slc26a9), partial CSB-YP021532XBF
CSB-EP021532XBF
CSB-BP021532XBF
CSB-MP021532XBF
CSB-EP021532XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein KIBRA (wwc1), partial CSB-YP026163XBF
CSB-EP026163XBF
CSB-BP026163XBF
CSB-MP026163XBF
CSB-EP026163XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Autophagy-related protein 13 (atg13), partial CSB-YP012203XBF
CSB-EP012203XBF
CSB-BP012203XBF
CSB-MP012203XBF
CSB-EP012203XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Germ cell-specific gene 1-like protein (gsg1l), partial CSB-YP009958XBF1
CSB-EP009958XBF1
CSB-BP009958XBF1
CSB-MP009958XBF1
CSB-EP009958XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis WD repeat-containing protein 37 (wdr37) CSB-YP026019XBF
CSB-EP026019XBF
CSB-BP026019XBF
CSB-MP026019XBF
CSB-EP026019XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Mediator of RNA polymerase II transcription subunit 28 (med28) CSB-YP013661XBF
CSB-EP013661XBF
CSB-BP013661XBF
CSB-MP013661XBF
CSB-EP013661XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Thymidylate kinase (tmk) CSB-YP007226GEH
CSB-EP007226GEH
CSB-BP007226GEH
CSB-MP007226GEH
CSB-EP007226GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans Elongation factor G (fusA), partial CSB-YP009373GEH
CSB-EP009373GEH
CSB-BP009373GEH
CSB-MP009373GEH
CSB-EP009373GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus thermodenitrificans tRNA pseudouridine synthase A (truA) CSB-YP019086GEH
CSB-EP019086GEH
CSB-BP019086GEH
CSB-MP019086GEH
CSB-EP019086GEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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