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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlorocebus aethiops WAP four-disulfide core domain protein 12 (WFDC12) CSB-YP026090DSU
CSB-EP026090DSU
CSB-BP026090DSU
CSB-MP026090DSU
CSB-EP026090DSU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mustela putorius furo Interferon gamma (IFNG) CSB-YP011050MOH
CSB-EP011050MOH
CSB-BP011050MOH
CSB-MP011050MOH
CSB-EP011050MOH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582CXG
CSB-EP009582CXG
CSB-BP009582CXG
CSB-MP009582CXG
CSB-EP009582CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Phosphoserine aminotransferase (serC) CSB-YP018838CXG
CSB-EP018838CXG
CSB-BP018838CXG
CSB-MP018838CXG
CSB-EP018838CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717CXG
CSB-EP009717CXG
CSB-BP009717CXG
CSB-MP009717CXG
CSB-EP009717CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Serine hydroxymethyltransferase (glyA) CSB-YP021274CXG
CSB-EP021274CXG
CSB-BP021274CXG
CSB-MP021274CXG
CSB-EP021274CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Peptide chain release factor 1 (prfA) CSB-YP015197CXG
CSB-EP015197CXG
CSB-BP015197CXG
CSB-MP015197CXG
CSB-EP015197CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Argininosuccinate synthase (argG) CSB-YP002234CXG
CSB-EP002234CXG
CSB-BP002234CXG
CSB-MP002234CXG
CSB-EP002234CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Triosephosphate isomerase (tpiA) CSB-YP024102CXG
CSB-EP024102CXG
CSB-BP024102CXG
CSB-MP024102CXG
CSB-EP024102CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Methionyl-tRNA formyltransferase (fmt) CSB-YP015150CXG
CSB-EP015150CXG
CSB-BP015150CXG
CSB-MP015150CXG
CSB-EP015150CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Translation initiation factor IF-2 (infB), partial CSB-YP015164CXG
CSB-EP015164CXG
CSB-BP015164CXG
CSB-MP015164CXG
CSB-EP015164CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Oligoribonuclease (orn) CSB-YP019586CXG
CSB-EP019586CXG
CSB-BP019586CXG
CSB-MP019586CXG
CSB-EP019586CXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Gamma-crystallin N (crygn) CSB-YP006024XBF
CSB-EP006024XBF
CSB-BP006024XBF
CSB-MP006024XBF
CSB-EP006024XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis ELAV-like protein 4 (elavl4) CSB-YP007595XBF
CSB-BP007595XBF
CSB-MP007595XBF
CSB-EP007595XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis RNA methyltransferase-like protein 1 (rnmtl1) CSB-YP019903XBF
CSB-EP019903XBF
CSB-BP019903XBF
CSB-MP019903XBF
CSB-EP019903XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Doublesex- and mab-3-related transcription factor A2 (dmrta2) CSB-YP006973XBF
CSB-EP006973XBF
CSB-BP006973XBF
CSB-MP006973XBF
CSB-EP006973XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Phytanoyl-CoA hydroxylase-interacting protein-like (phyhipl) CSB-YP017949DIL
CSB-EP017949DIL
CSB-BP017949DIL
CSB-MP017949DIL
CSB-EP017949DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae NADH-cytochrome b5 reductase 2 (MCR1), partial CSB-YP006319MPM1
CSB-EP006319MPM1
CSB-BP006319MPM1
CSB-MP006319MPM1
CSB-EP006319MPM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Mitotic-spindle organizing protein 1 (MGG_14000) CSB-YP003125MPM
CSB-EP003125MPM
CSB-BP003125MPM
CSB-MP003125MPM
CSB-EP003125MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Magnaporthe oryzae Eukaryotic translation initiation factor 3 subunit F (MGG_10653) CSB-YP007535MPM
CSB-EP007535MPM
CSB-BP007535MPM
CSB-MP007535MPM
CSB-EP007535MPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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