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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermotoga petrophila 6-phosphofructokinase (pfkA) CSB-YP017822TKF
CSB-EP017822TKF
CSB-BP017822TKF
CSB-MP017822TKF
CSB-EP017822TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Peptide deformylase (def) CSB-YP017707TKF
CSB-EP017707TKF
CSB-BP017707TKF
CSB-MP017707TKF
CSB-EP017707TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lipoyl synthase (lipA) CSB-YP012927SUJ
CSB-EP012927SUJ
CSB-BP012927SUJ
CSB-MP012927SUJ
CSB-EP012927SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease 3 (rnc) CSB-YP019809SUJ
CSB-EP019809SUJ
CSB-BP019809SUJ
CSB-MP019809SUJ
CSB-EP019809SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor Ts (tsf) CSB-YP025124SUJ
CSB-EP025124SUJ
CSB-BP025124SUJ
CSB-MP025124SUJ
CSB-EP025124SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 6-phosphofructokinase (pfkA) CSB-YP017822SUJ
CSB-EP017822SUJ
CSB-BP017822SUJ
CSB-MP017822SUJ
CSB-EP017822SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium vivax 40S ribosomal protein S3a (PVX_095080) CSB-YP020444EWW
CSB-EP020444EWW
CSB-BP020444EWW
CSB-MP020444EWW
CSB-EP020444EWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146CWX
CSB-EP019146CWX
CSB-BP019146CWX
CSB-MP019146CWX
CSB-EP019146CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri GTPase Era (era) CSB-YP007759CWX
CSB-EP007759CWX
CSB-BP007759CWX
CSB-MP007759CWX
CSB-EP007759CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Mini-chromosome maintenance complex-binding protein (MCMBP), partial CSB-YP002918BO
CSB-EP002918BO
CSB-BP002918BO
CSB-MP002918BO
CSB-EP002918BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Transcription elongation factor A N-terminal and central domain-containing protein 2 (TCEANC2) CSB-YP023280BO
CSB-EP023280BO
CSB-BP023280BO
CSB-MP023280BO
CSB-EP023280BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio TM2 domain-containing protein 3 (tm2d3), partial CSB-YP023614DIL1
CSB-EP023614DIL1
CSB-BP023614DIL1
CSB-MP023614DIL1
CSB-EP023614DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Nucleoredoxin-like protein 1 (nxnl1) CSB-YP016225DIL
CSB-EP016225DIL
CSB-BP016225DIL
CSB-MP016225DIL
CSB-EP016225DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein FAM151A (fam151a), partial CSB-YP008087DIL1
CSB-EP008087DIL1
CSB-BP008087DIL1
CSB-MP008087DIL1
CSB-EP008087DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Malate dehydrogenase (mdh) CSB-YP013623MON
CSB-EP013623MON
CSB-BP013623MON
CSB-MP013623MON
CSB-EP013623MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824MON
CSB-EP005824MON
CSB-BP005824MON
CSB-MP005824MON
CSB-EP005824MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Glycine cleavage system H protein (gcvH) CSB-YP009335MON
CSB-EP009335MON
CSB-BP009335MON
CSB-MP009335MON
CSB-EP009335MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582MON
CSB-EP009582MON
CSB-BP009582MON
CSB-MP009582MON
CSB-EP009582MON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae tRNA dimethylallyltransferase (miaA) CSB-YP024529HSD
CSB-EP024529HSD
CSB-BP024529HSD
CSB-MP024529HSD
CSB-EP024529HSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Ribonuclease 3 (rnc) CSB-YP019809HSD
CSB-EP019809HSD
CSB-BP019809HSD
CSB-MP019809HSD
CSB-EP019809HSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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