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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio Histone-lysine N-methyltransferase PRDM9 (prdm9), partial CSB-YP750787DIL
CSB-EP750787DIL
CSB-BP750787DIL
CSB-MP750787DIL
CSB-EP750787DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein phosphatase 4 regulatory subunit 3A (Smek1), partial CSB-YP750788MO
CSB-EP750788MO
CSB-BP750788MO
CSB-MP750788MO
CSB-EP750788MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Trimeric intracellular cation channel type A (tmem38a), partial CSB-YP750789DIL1
CSB-EP750789DIL1
CSB-BP750789DIL1
CSB-MP750789DIL1
CSB-EP750789DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis DnaJ homolog subfamily C member 2 (dnajc2), partial CSB-YP750790XBF
CSB-EP750790XBF
CSB-BP750790XBF
CSB-MP750790XBF
CSB-EP750790XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis GDP-Man:Man (3)GlcNAc (2)-PP-Dol alpha-1,2-mannosyltransferase (alg11), partial CSB-YP750791XBF1
CSB-EP750791XBF1
CSB-BP750791XBF1
CSB-MP750791XBF1
CSB-EP750791XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Diacylglycerol O-acyltransferase 2 (dgat2), partial CSB-YP750792XBF1
CSB-EP750792XBF1
CSB-BP750792XBF1
CSB-MP750792XBF1
CSB-EP750792XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Actin, cytoplasmic 2 (actg1) CSB-YP750793XBF
CSB-EP750793XBF
CSB-BP750793XBF
CSB-MP750793XBF
CSB-EP750793XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse ADP-ribosylation factor-like protein 11 (Arl11) CSB-YP750794MO
CSB-EP750794MO
CSB-BP750794MO
CSB-MP750794MO
CSB-EP750794MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Transcription factor Spi-C (Spic) CSB-YP750795MO
CSB-EP750795MO
CSB-BP750795MO
CSB-MP750795MO
CSB-EP750795MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Collagen type IV alpha-3-binding protein (col4a3bp), partial CSB-YP750796XBF
CSB-EP750796XBF
CSB-BP750796XBF
CSB-MP750796XBF
CSB-EP750796XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Neuroblastoma breakpoint family member 10 (NBPF10), partial CSB-YP750797HU
CSB-EP750797HU
CSB-BP750797HU
CSB-MP750797HU
CSB-EP750797HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Centrosomal protein of 89 kDa (cep89), partial CSB-YP750798DIL
CSB-EP750798DIL
CSB-BP750798DIL
CSB-MP750798DIL
CSB-EP750798DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phospholipase B-like 1 (PLBD1) CSB-YP750799HU
CSB-EP750799HU
CSB-BP750799HU
CSB-MP750799HU
CSB-EP750799HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Rho GTPase-activating protein 11A (ARHGAP11A), partial CSB-YP750800HU
CSB-EP750800HU
CSB-BP750800HU
CSB-MP750800HU
CSB-EP750800HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Hepatoma-derived growth factor-related protein 2 (hdgfrp2), partial CSB-YP750801XBF
CSB-EP750801XBF
CSB-BP750801XBF
CSB-MP750801XBF
CSB-EP750801XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine protease inhibitor A3A (Serpina3a) CSB-YP750802MO
CSB-EP750802MO
CSB-BP750802MO
CSB-MP750802MO
CSB-EP750802MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein kinase SIK3 (Sik3), partial CSB-YP750803MO
CSB-EP750803MO
CSB-BP750803MO
CSB-MP750803MO
CSB-EP750803MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Threonylcarbamoyladenosine tRNA methylthiotransferase (cdkal1), partial CSB-YP750804XBF1
CSB-EP750804XBF1
CSB-BP750804XBF1
CSB-MP750804XBF1
CSB-EP750804XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Lysosomal-associated transmembrane protein 4A (Laptm4a), partial CSB-YP750805RA1
CSB-EP750805RA1
CSB-BP750805RA1
CSB-MP750805RA1
CSB-EP750805RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein limb expression 1 homolog (Lix1) CSB-YP750806MO
CSB-EP750806MO
CSB-BP750806MO
CSB-MP750806MO
CSB-EP750806MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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