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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Uracil phosphoribosyltransferase (upp) CSB-YP750707CQF
CSB-EP750707CQF
CSB-BP750707CQF
CSB-MP750707CQF
CSB-EP750707CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized transporter DIP0570 (DIP0570), partial CSB-YP750708CQF
CSB-EP750708CQF
CSB-BP750708CQF
CSB-MP750708CQF
CSB-EP750708CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP750709CQF
CSB-EP750709CQF
CSB-BP750709CQF
CSB-MP750709CQF
CSB-EP750709CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S8 (rpsH) CSB-YP750710CQF
CSB-EP750710CQF
CSB-BP750710CQF
CSB-MP750710CQF
CSB-EP750710CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S10 (rpsJ) CSB-YP750711CQF
CSB-EP750711CQF
CSB-BP750711CQF
CSB-MP750711CQF
CSB-EP750711CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphobilinogen deaminase (hemC) CSB-YP750712CQF
CSB-EP750712CQF
CSB-BP750712CQF
CSB-MP750712CQF
CSB-EP750712CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP750713CQF
CSB-EP750713CQF
CSB-BP750713CQF
CSB-MP750713CQF
CSB-EP750713CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 15 (CRRSP15) CSB-YP750714DOA
CSB-EP750714DOA
CSB-BP750714DOA
CSB-MP750714DOA
CSB-EP750714DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable receptor-like serine/threonine-protein kinase At4g34500 (At4g34500), partial CSB-YP750715DOA1
CSB-EP750715DOA1
CSB-BP750715DOA1
CSB-MP750715DOA1
CSB-EP750715DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphoinositide phospholipase C 9 (PLC9), partial CSB-YP750716DOA
CSB-EP750716DOA
CSB-BP750716DOA
CSB-MP750716DOA
CSB-EP750716DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable glucuronoxylan glucuronosyltransferase F8H (F8H), partial CSB-YP750717DOA
CSB-EP750717DOA
CSB-BP750717DOA
CSB-MP750717DOA
CSB-EP750717DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster MOXD1 homolog 2 (olf413), partial CSB-YP750718DLU1
CSB-EP750718DLU1
CSB-BP750718DLU1
CSB-MP750718DLU1
CSB-EP750718DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Thioredoxin Y1, chloroplastic (At1g76760) CSB-YP750719DOA
CSB-EP750719DOA
CSB-BP750719DOA
CSB-MP750719DOA
CSB-EP750719DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NADPH-dependent diflavin oxidoreductase ATR3 (ATR3), partial CSB-YP750720DOA
CSB-EP750720DOA
CSB-BP750720DOA
CSB-MP750720DOA
CSB-EP750720DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-1,3-galactosyltransferase 7 (B3GALT7), partial CSB-YP750721DOA
CSB-EP750721DOA
CSB-BP750721DOA
CSB-MP750721DOA
CSB-EP750721DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Uncharacterized protein KIAA0090 homolog, partial CSB-YP750722XBE
CSB-EP750722XBE
CSB-BP750722XBE
CSB-MP750722XBE
CSB-EP750722XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Exonuclease 3'-5' domain-containing protein 1 (exd1) CSB-YP750723XBE
CSB-EP750723XBE
CSB-BP750723XBE
CSB-MP750723XBE
CSB-EP750723XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis SOSS complex subunit B1-B (obfc2b-b) CSB-YP750724XBE
CSB-EP750724XBE
CSB-BP750724XBE
CSB-MP750724XBE
CSB-EP750724XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Kelch-like protein 12 (klhl12), partial CSB-YP750725XBE
CSB-EP750725XBE
CSB-BP750725XBE
CSB-MP750725XBE
CSB-EP750725XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ankyrin repeat domain-containing protein SOWAHB (sowahb), partial CSB-YP750726XBE
CSB-EP750726XBE
CSB-BP750726XBE
CSB-MP750726XBE
CSB-EP750726XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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