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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio COP9 signalosome complex subunit 5 (cops5) CSB-YP750847DIL
CSB-EP750847DIL
CSB-BP750847DIL
CSB-MP750847DIL
CSB-EP750847DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein C20orf11 homolog (zgc:73100) CSB-YP750848DIL
CSB-EP750848DIL
CSB-BP750848DIL
CSB-MP750848DIL
CSB-EP750848DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Abhydrolase domain-containing protein FAM108C1 (FAM108C1) CSB-YP750849HU
CSB-EP750849HU
CSB-BP750849HU
CSB-MP750849HU
CSB-EP750849HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ubiquitin-conjugating enzyme E2 Z (ube2z) CSB-YP750850XBE
CSB-EP750850XBE
CSB-BP750850XBE
CSB-MP750850XBE
CSB-EP750850XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Lysosomal thioesterase PPT2-A (ppt2-a) CSB-YP750851XBE
CSB-EP750851XBE
CSB-BP750851XBE
CSB-MP750851XBE
CSB-EP750851XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Lysine-specific demethylase 3A (Kdm3a), partial CSB-YP750852MO
CSB-EP750852MO
CSB-BP750852MO
CSB-MP750852MO
CSB-EP750852MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (Ndufv3) CSB-YP750853RA
CSB-EP750853RA
CSB-BP750853RA
CSB-MP750853RA
CSB-EP750853RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RNA polymerase-associated protein CTR9 homolog (CTR9), partial CSB-YP750854HU
CSB-EP750854HU
CSB-BP750854HU
CSB-MP750854HU
CSB-EP750854HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase MINDY-2 (Mindy2) CSB-YP750855MO
CSB-BP750855MO
CSB-MP750855MO
CSB-EP750855MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Myosin light chain kinase, smooth muscle (Mylk), partial CSB-YP750856MO
CSB-EP750856MO
CSB-BP750856MO
CSB-MP750856MO
CSB-EP750856MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 39S ribosomal protein L17, mitochondrial (Mrpl17) CSB-YP750857RA
CSB-EP750857RA
CSB-BP750857RA
CSB-MP750857RA
CSB-EP750857RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Elongation factor 1-gamma (eef1g) CSB-YP750858DIL
CSB-EP750858DIL
CSB-BP750858DIL
CSB-MP750858DIL
CSB-EP750858DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Coiled-coil domain-containing protein 127 (Ccdc127) CSB-YP750859RA
CSB-EP750859RA
CSB-BP750859RA
CSB-MP750859RA
CSB-EP750859RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse GRAM domain-containing protein 3 (Gramd3) CSB-YP750860MO
CSB-EP750860MO
CSB-BP750860MO
CSB-MP750860MO
CSB-EP750860MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein ATPAF1-AS1 (ATPAF1-AS1), partial CSB-YP750861HU1
CSB-EP750861HU1
CSB-BP750861HU1
CSB-MP750861HU1
CSB-EP750861HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Vesicular inhibitory amino acid transporter (slc32a1), partial CSB-YP750862XBE
CSB-EP750862XBE
CSB-BP750862XBE
CSB-MP750862XBE
CSB-EP750862XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 2 (Lrit2), partial CSB-YP750863MO1
CSB-EP750863MO1
CSB-BP750863MO1
CSB-MP750863MO1
CSB-EP750863MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Neuroguidin (ngdn) CSB-YP750864DIL
CSB-EP750864DIL
CSB-BP750864DIL
CSB-MP750864DIL
CSB-EP750864DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Mediator of RNA polymerase II transcription subunit 27 (med27) CSB-YP750865DIL
CSB-EP750865DIL
CSB-BP750865DIL
CSB-MP750865DIL
CSB-EP750865DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 (jmjd6) CSB-YP750866DIL
CSB-EP750866DIL
CSB-BP750866DIL
CSB-MP750866DIL
CSB-EP750866DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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