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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio vulnificus Transcription elongation factor GreB (greB) CSB-YP752254VCQ
CSB-EP752254VCQ
CSB-BP752254VCQ
CSB-MP752254VCQ
CSB-EP752254VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Probable GTP-binding protein EngB (engB) CSB-YP752255VCQ
CSB-EP752255VCQ
CSB-BP752255VCQ
CSB-MP752255VCQ
CSB-EP752255VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus tRNA (uracil (54)-C (5))-methyltransferase (trmA) CSB-YP752256VCQ
CSB-EP752256VCQ
CSB-BP752256VCQ
CSB-MP752256VCQ
CSB-EP752256VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Diaminopimelate epimerase (dapF) CSB-YP752257VCQ
CSB-EP752257VCQ
CSB-BP752257VCQ
CSB-MP752257VCQ
CSB-EP752257VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus DNA replication and repair protein recF (recF) CSB-YP752258VCQ
CSB-EP752258VCQ
CSB-BP752258VCQ
CSB-MP752258VCQ
CSB-EP752258VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0324 membrane protein WS2204 (WS2204), partial CSB-YP752259WBQ
CSB-EP752259WBQ
CSB-BP752259WBQ
CSB-MP752259WBQ
CSB-EP752259WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S6 (rpsF) CSB-YP752260WBQ
CSB-EP752260WBQ
CSB-BP752260WBQ
CSB-MP752260WBQ
CSB-EP752260WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative membrane protein insertion efficiency factor (WS1287) CSB-YP752261WBQ
CSB-EP752261WBQ
CSB-BP752261WBQ
CSB-MP752261WBQ
CSB-EP752261WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP752262WBQ
CSB-EP752262WBQ
CSB-BP752262WBQ
CSB-MP752262WBQ
CSB-EP752262WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP752263WBQ
CSB-EP752263WBQ
CSB-BP752263WBQ
CSB-MP752263WBQ
CSB-EP752263WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP752264WBQ
CSB-EP752264WBQ
CSB-BP752264WBQ
CSB-MP752264WBQ
CSB-EP752264WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Putative Holliday junction resolvase (PG_2202) CSB-YP752265EYA
CSB-EP752265EYA
CSB-BP752265EYA
CSB-MP752265EYA
CSB-EP752265EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis GTPase Der (der) CSB-YP752266EYA
CSB-EP752266EYA
CSB-BP752266EYA
CSB-MP752266EYA
CSB-EP752266EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP752267EYA
CSB-EP752267EYA
CSB-BP752267EYA
CSB-MP752267EYA
CSB-EP752267EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP752268EYA
CSB-EP752268EYA
CSB-BP752268EYA
CSB-MP752268EYA
CSB-EP752268EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Elongation factor G (fusA), partial CSB-YP752269EYA
CSB-EP752269EYA
CSB-BP752269EYA
CSB-MP752269EYA
CSB-EP752269EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 50S ribosomal protein L16 (rplP) CSB-YP752270EYA
CSB-EP752270EYA
CSB-BP752270EYA
CSB-MP752270EYA
CSB-EP752270EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 30S ribosomal protein S5 (rpsE) CSB-YP752271EYA
CSB-EP752271EYA
CSB-BP752271EYA
CSB-MP752271EYA
CSB-EP752271EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 30S ribosomal protein S4 (rpsD) CSB-YP752272EYA
CSB-EP752272EYA
CSB-BP752272EYA
CSB-MP752272EYA
CSB-EP752272EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis UPF0102 protein PG_1874 (PG_1874) CSB-YP752273EYA
CSB-EP752273EYA
CSB-BP752273EYA
CSB-MP752273EYA
CSB-EP752273EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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