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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chromobacterium violaceum Peptidyl-tRNA hydrolase (pth) CSB-YP752454CKA
CSB-EP752454CKA
CSB-BP752454CKA
CSB-MP752454CKA
CSB-EP752454CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum DNA gyrase inhibitor YacG (yacG) CSB-YP752455CKA
CSB-EP752455CKA
CSB-BP752455CKA
CSB-MP752455CKA
CSB-EP752455CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S21 (rpsU) CSB-YP752456CKA
CSB-EP752456CKA
CSB-BP752456CKA
CSB-MP752456CKA
CSB-EP752456CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Deoxyribose-phosphate aldolase (deoC) CSB-YP752457CKA
CSB-EP752457CKA
CSB-BP752457CKA
CSB-MP752457CKA
CSB-EP752457CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Putative iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP752458CKA
CSB-EP752458CKA
CSB-BP752458CKA
CSB-MP752458CKA
CSB-EP752458CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum DNA mismatch repair protein MutS (mutS), partial CSB-YP752459CKA
CSB-EP752459CKA
CSB-BP752459CKA
CSB-MP752459CKA
CSB-EP752459CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Shikimate dehydrogenase (aroE) CSB-YP752460CKA
CSB-EP752460CKA
CSB-BP752460CKA
CSB-MP752460CKA
CSB-EP752460CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP752461CKA
CSB-EP752461CKA
CSB-BP752461CKA
CSB-MP752461CKA
CSB-EP752461CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Probable acetoacetate decarboxylase (adc) CSB-YP752462CKA
CSB-EP752462CKA
CSB-BP752462CKA
CSB-MP752462CKA
CSB-EP752462CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Cell division protein FtsB (ftsB), partial CSB-YP752463CKA
CSB-EP752463CKA
CSB-BP752463CKA
CSB-MP752463CKA
CSB-EP752463CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP752464CKA
CSB-EP752464CKA
CSB-BP752464CKA
CSB-MP752464CKA
CSB-EP752464CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Probable septum site-determining protein MinC (minC) CSB-YP752465CKA
CSB-EP752465CKA
CSB-BP752465CKA
CSB-MP752465CKA
CSB-EP752465CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Adenylate kinase (adk) CSB-YP752466CKA
CSB-EP752466CKA
CSB-BP752466CKA
CSB-MP752466CKA
CSB-EP752466CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum tRNA (Ile)-lysidine synthase (tilS) CSB-YP752467CKA
CSB-EP752467CKA
CSB-BP752467CKA
CSB-MP752467CKA
CSB-EP752467CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Lipoyl synthase (lipA) CSB-YP752468CKA
CSB-EP752468CKA
CSB-BP752468CKA
CSB-MP752468CKA
CSB-EP752468CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP752469CKA
CSB-EP752469CKA
CSB-BP752469CKA
CSB-MP752469CKA
CSB-EP752469CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum NADH pyrophosphatase (nudC) CSB-YP752470CKA
CSB-EP752470CKA
CSB-BP752470CKA
CSB-MP752470CKA
CSB-EP752470CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Taurine import ATP-binding protein TauB (tauB) CSB-YP752471CKA
CSB-EP752471CKA
CSB-BP752471CKA
CSB-MP752471CKA
CSB-EP752471CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum NADH-quinone oxidoreductase subunit B 2 (nuoB2) CSB-YP752472CKA
CSB-EP752472CKA
CSB-BP752472CKA
CSB-MP752472CKA
CSB-EP752472CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Valine--tRNA ligase (valS), partial CSB-YP752473CKA
CSB-EP752473CKA
CSB-BP752473CKA
CSB-MP752473CKA
CSB-EP752473CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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