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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Onion yellows phytoplasma Thymidine kinase (tdk) CSB-YP751110OBN
CSB-EP751110OBN
CSB-BP751110OBN
CSB-MP751110OBN
CSB-EP751110OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable potassium transporter 4 (HAK4), partial CSB-YP751111OFG
CSB-EP751111OFG
CSB-BP751111OFG
CSB-MP751111OFG
CSB-EP751111OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (GLO5) CSB-YP751112OFG
CSB-EP751112OFG
CSB-BP751112OFG
CSB-MP751112OFG
CSB-EP751112OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable cellulose synthase A catalytic subunit 6 [UDP-forming] (CESA6), partial CSB-YP751113OFG
CSB-EP751113OFG
CSB-BP751113OFG
CSB-MP751113OFG
CSB-EP751113OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cytokinin dehydrogenase 7 (CKX7), partial CSB-YP751114OFG
CSB-EP751114OFG
CSB-BP751114OFG
CSB-MP751114OFG
CSB-EP751114OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Double-stranded RNA-binding protein 4 (DRB4) CSB-YP751115OFG
CSB-EP751115OFG
CSB-BP751115OFG
CSB-MP751115OFG
CSB-EP751115OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP751116EVD
CSB-EP751116EVD
CSB-BP751116EVD
CSB-MP751116EVD
CSB-EP751116EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP751117EVD
CSB-EP751117EVD
CSB-BP751117EVD
CSB-MP751117EVD
CSB-EP751117EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Photosystem I reaction center subunit IX (psaJ), partial CSB-YP751118EVD
CSB-EP751118EVD
CSB-BP751118EVD
CSB-MP751118EVD
CSB-EP751118EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Cytochrome b6 (petB), partial CSB-YP751119EVD1
CSB-EP751119EVD1
CSB-BP751119EVD1
CSB-MP751119EVD1
CSB-EP751119EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP751120EVD
CSB-EP751120EVD
CSB-BP751120EVD
CSB-MP751120EVD
CSB-EP751120EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP751121EVD
CSB-EP751121EVD
CSB-BP751121EVD
CSB-MP751121EVD
CSB-EP751121EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (chlL) CSB-YP751122EVD
CSB-EP751122EVD
CSB-BP751122EVD
CSB-MP751122EVD
CSB-EP751122EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable histone acetyltransferase HAC-like 1 (Os02g0137500, LOC_Os02g04490), partial CSB-YP751123OFG
CSB-EP751123OFG
CSB-BP751123OFG
CSB-MP751123OFG
CSB-EP751123OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative vacuolar cation/proton exchanger 4 (Os02g0138900, LOC_Os02g04630), partial CSB-YP751124OFG
CSB-EP751124OFG
CSB-BP751124OFG
CSB-MP751124OFG
CSB-EP751124OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Expansin-A32 (EXPA32) CSB-YP751125OFG
CSB-EP751125OFG
CSB-BP751125OFG
CSB-MP751125OFG
CSB-EP751125OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Germin-like protein 8-2 (GER3) CSB-YP751126OFG
CSB-EP751126OFG
CSB-BP751126OFG
CSB-MP751126OFG
CSB-EP751126OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative aconitate hydratase, cytoplasmic (Os08g0191100, LOC_Os08g09200), partial CSB-YP751127OFG
CSB-EP751127OFG
CSB-BP751127OFG
CSB-MP751127OFG
CSB-EP751127OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Actin-related protein 2 (ARP2) CSB-YP751128OFG
CSB-EP751128OFG
CSB-BP751128OFG
CSB-MP751128OFG
CSB-EP751128OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Hexokinase-4, chloroplastic (HXK4) CSB-YP751129OFG
CSB-EP751129OFG
CSB-BP751129OFG
CSB-MP751129OFG
CSB-EP751129OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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