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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria monocytogenes serotype 4b Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP751311LAAD
CSB-EP751311LAAD
CSB-BP751311LAAD
CSB-MP751311LAAD
CSB-EP751311LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Chaperone protein DnaJ (dnaJ) CSB-YP751312LAAD
CSB-EP751312LAAD
CSB-BP751312LAAD
CSB-MP751312LAAD
CSB-EP751312LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b DNA repair protein recO (recO) CSB-YP751313LAAD
CSB-EP751313LAAD
CSB-BP751313LAAD
CSB-MP751313LAAD
CSB-EP751313LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP751314LAAD
CSB-EP751314LAAD
CSB-BP751314LAAD
CSB-MP751314LAAD
CSB-EP751314LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b DNA mismatch repair protein mutL (mutL), partial CSB-YP751315LAAD
CSB-EP751315LAAD
CSB-BP751315LAAD
CSB-MP751315LAAD
CSB-EP751315LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Probable butyrate kinase (buk) CSB-YP751316LAAD
CSB-EP751316LAAD
CSB-BP751316LAAD
CSB-MP751316LAAD
CSB-EP751316LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Undecaprenyl pyrophosphate synthase (uppS) CSB-YP751317LAAD
CSB-EP751317LAAD
CSB-BP751317LAAD
CSB-MP751317LAAD
CSB-EP751317LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0154 protein LMOf2365_1324 (LMOf2365_1324), partial CSB-YP751318LAAD
CSB-EP751318LAAD
CSB-BP751318LAAD
CSB-MP751318LAAD
CSB-EP751318LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP751319LAAD
CSB-EP751319LAAD
CSB-BP751319LAAD
CSB-MP751319LAAD
CSB-EP751319LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b MutS2 protein (mutS2), partial CSB-YP751320LAAD
CSB-EP751320LAAD
CSB-BP751320LAAD
CSB-MP751320LAAD
CSB-EP751320LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Cobyric acid synthase (cobQ), partial CSB-YP751321LAAD
CSB-EP751321LAAD
CSB-BP751321LAAD
CSB-MP751321LAAD
CSB-EP751321LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Cobalamin synthase (cobS), partial CSB-YP751322LAAD1
CSB-EP751322LAAD1
CSB-BP751322LAAD1
CSB-MP751322LAAD1
CSB-EP751322LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 (ispD2) CSB-YP751323LAAD
CSB-EP751323LAAD
CSB-BP751323LAAD
CSB-MP751323LAAD
CSB-EP751323LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP751324LAAD
CSB-EP751324LAAD
CSB-BP751324LAAD
CSB-MP751324LAAD
CSB-EP751324LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Ribose-5-phosphate isomerase A (rpiA) CSB-YP751325LAAD
CSB-EP751325LAAD
CSB-BP751325LAAD
CSB-MP751325LAAD
CSB-EP751325LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glucosamine-6-phosphate deaminase (nagB) CSB-YP751326LAAD
CSB-EP751326LAAD
CSB-BP751326LAAD
CSB-MP751326LAAD
CSB-EP751326LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Pantothenate kinase (coaA) CSB-YP751327LAAD
CSB-EP751327LAAD
CSB-BP751327LAAD
CSB-MP751327LAAD
CSB-EP751327LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Protein psiE homolog (psiE), partial CSB-YP751328LAAD1
CSB-EP751328LAAD1
CSB-BP751328LAAD1
CSB-MP751328LAAD1
CSB-EP751328LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP751329LAAD
CSB-EP751329LAAD
CSB-BP751329LAAD
CSB-MP751329LAAD
CSB-EP751329LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Protein translocase subunit SecA 2 (secA2), partial CSB-YP751330LAAD
CSB-EP751330LAAD
CSB-BP751330LAAD
CSB-MP751330LAAD
CSB-EP751330LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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