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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aeromonas salmonicida ATP synthase subunit beta (atpD) CSB-YP002350AUI
CSB-EP002350AUI
CSB-BP002350AUI
CSB-MP002350AUI
CSB-EP002350AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida ATP synthase subunit b (atpF), partial CSB-YP002358AUI1
CSB-EP002358AUI1
CSB-BP002358AUI1
CSB-MP002358AUI1
CSB-EP002358AUI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. ATP synthase subunit alpha (atpA), partial CSB-YP002344PYU
CSB-EP002344PYU
CSB-BP002344PYU
CSB-MP002344PYU
CSB-EP002344PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Elongation factor G (fusA), partial CSB-YP009373PYU
CSB-EP009373PYU
CSB-BP009373PYU
CSB-MP009373PYU
CSB-EP009373PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Thymidylate synthase (thyA) CSB-YP025393PYU
CSB-EP025393PYU
CSB-BP025393PYU
CSB-MP025393PYU
CSB-EP025393PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Malate dehydrogenase (mdh) CSB-YP013623PYU
CSB-EP013623PYU
CSB-BP013623PYU
CSB-MP013623PYU
CSB-EP013623PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Translation initiation factor IF-2 (infB), partial CSB-YP015164PYU
CSB-EP015164PYU
CSB-BP015164PYU
CSB-MP015164PYU
CSB-EP015164PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917PYU
CSB-EP022917PYU
CSB-BP022917PYU
CSB-MP022917PYU
CSB-EP022917PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Adenosylhomocysteinase (ahcY) CSB-YP001474PYU
CSB-EP001474PYU
CSB-BP001474PYU
CSB-MP001474PYU
CSB-EP001474PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter sp. Methionyl-tRNA formyltransferase (fmt) CSB-YP015150PYU
CSB-EP015150PYU
CSB-BP015150PYU
CSB-MP015150PYU
CSB-EP015150PYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717MOK
CSB-EP009717MOK
CSB-BP009717MOK
CSB-MP009717MOK
CSB-EP009717MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum ATP synthase subunit b (atpF), partial CSB-YP002358MOK1
CSB-EP002358MOK1
CSB-BP002358MOK1
CSB-MP002358MOK1
CSB-EP002358MOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Triosephosphate isomerase (tpiA) CSB-YP024102MOK
CSB-EP024102MOK
CSB-BP024102MOK
CSB-MP024102MOK
CSB-EP024102MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium gilvum Thymidylate synthase (thyA) CSB-YP025393MOK
CSB-EP025393MOK
CSB-BP025393MOK
CSB-MP025393MOK
CSB-EP025393MOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Methionyl-tRNA formyltransferase (fmt) CSB-YP015150YAL
CSB-EP015150YAL
CSB-BP015150YAL
CSB-MP015150YAL
CSB-EP015150YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Peptide deformylase (def) CSB-YP017707YAL
CSB-EP017707YAL
CSB-BP017707YAL
CSB-MP017707YAL
CSB-EP017707YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717YAL
CSB-EP009717YAL
CSB-BP009717YAL
CSB-MP009717YAL
CSB-EP009717YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Cysteine desulfurase (sufS) CSB-YP015769YAL
CSB-EP015769YAL
CSB-BP015769YAL
CSB-MP015769YAL
CSB-EP015769YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Peptide chain release factor 1 (prfA) CSB-YP015197YAL
CSB-EP015197YAL
CSB-BP015197YAL
CSB-MP015197YAL
CSB-EP015197YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-tubulin N-acetyltransferase (LbrM25_V2.1130, LbrM_25_1130) CSB-YP004059LOC
CSB-EP004059LOC
CSB-BP004059LOC
CSB-MP004059LOC
CSB-EP004059LOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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