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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio vulnificus Ribose-phosphate pyrophosphokinase (prs) CSB-YP752234VCQ
CSB-EP752234VCQ
CSB-BP752234VCQ
CSB-MP752234VCQ
CSB-EP752234VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0250 protein VV0902 (VV0902) CSB-YP752235VCQ
CSB-EP752235VCQ
CSB-BP752235VCQ
CSB-MP752235VCQ
CSB-EP752235VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus tRNA sulfurtransferase (thiI) CSB-YP752236VCQ
CSB-EP752236VCQ
CSB-BP752236VCQ
CSB-MP752236VCQ
CSB-EP752236VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus SsrA-binding protein (smpB) CSB-YP752237VCQ
CSB-EP752237VCQ
CSB-BP752237VCQ
CSB-MP752237VCQ
CSB-EP752237VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Co-chaperone protein hscB homolog (hscB) CSB-YP752238VCQ
CSB-EP752238VCQ
CSB-BP752238VCQ
CSB-MP752238VCQ
CSB-EP752238VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Recombination-associated protein RdgC (rdgC) CSB-YP752239VCQ
CSB-EP752239VCQ
CSB-BP752239VCQ
CSB-MP752239VCQ
CSB-EP752239VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Thymidylate synthase (thyA) CSB-YP752240VCQ
CSB-EP752240VCQ
CSB-BP752240VCQ
CSB-MP752240VCQ
CSB-EP752240VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP752241VCQ
CSB-EP752241VCQ
CSB-BP752241VCQ
CSB-MP752241VCQ
CSB-EP752241VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP752242VCQ
CSB-EP752242VCQ
CSB-BP752242VCQ
CSB-MP752242VCQ
CSB-EP752242VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Bifunctional protein HldE (hldE) CSB-YP752243VCQ
CSB-EP752243VCQ
CSB-BP752243VCQ
CSB-MP752243VCQ
CSB-EP752243VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Deoxyribose-phosphate aldolase 1 (deoC1) CSB-YP752244VCQ
CSB-EP752244VCQ
CSB-BP752244VCQ
CSB-MP752244VCQ
CSB-EP752244VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Chaperone surA (surA) CSB-YP752245VCQ
CSB-EP752245VCQ
CSB-BP752245VCQ
CSB-MP752245VCQ
CSB-EP752245VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L21 (rplU) CSB-YP752246VCQ
CSB-EP752246VCQ
CSB-BP752246VCQ
CSB-MP752246VCQ
CSB-EP752246VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Inorganic pyrophosphatase (ppa) CSB-YP752247VCQ
CSB-EP752247VCQ
CSB-BP752247VCQ
CSB-MP752247VCQ
CSB-EP752247VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP752248VCQ
CSB-EP752248VCQ
CSB-BP752248VCQ
CSB-MP752248VCQ
CSB-EP752248VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 30S ribosomal protein S5 (rpsE) CSB-YP752249VCQ
CSB-EP752249VCQ
CSB-BP752249VCQ
CSB-MP752249VCQ
CSB-EP752249VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 30S ribosomal protein S17 (rpsQ) CSB-YP752250VCQ
CSB-EP752250VCQ
CSB-BP752250VCQ
CSB-MP752250VCQ
CSB-EP752250VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L3 (rplC) CSB-YP752251VCQ
CSB-EP752251VCQ
CSB-BP752251VCQ
CSB-MP752251VCQ
CSB-EP752251VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphopantetheine adenylyltransferase (coaD) CSB-YP752252VCQ
CSB-EP752252VCQ
CSB-BP752252VCQ
CSB-MP752252VCQ
CSB-EP752252VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Orotate phosphoribosyltransferase (pyrE) CSB-YP752253VCQ
CSB-EP752253VCQ
CSB-BP752253VCQ
CSB-MP752253VCQ
CSB-EP752253VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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