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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii L-seryl-tRNA (Sec) selenium transferase (selA) CSB-YP752294PIJ
CSB-EP752294PIJ
CSB-BP752294PIJ
CSB-MP752294PIJ
CSB-EP752294PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Phosphopantetheine adenylyltransferase (coaD) CSB-YP752295PIJ
CSB-EP752295PIJ
CSB-BP752295PIJ
CSB-MP752295PIJ
CSB-EP752295PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Glycerol kinase (glpK), partial CSB-YP752296PIJ
CSB-EP752296PIJ
CSB-BP752296PIJ
CSB-MP752296PIJ
CSB-EP752296PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Met repressor (metJ) CSB-YP752297PIJ
CSB-EP752297PIJ
CSB-BP752297PIJ
CSB-MP752297PIJ
CSB-EP752297PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Glutamate racemase (murI) CSB-YP752298PIJ
CSB-EP752298PIJ
CSB-BP752298PIJ
CSB-MP752298PIJ
CSB-EP752298PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S19 (rpsS) CSB-YP752299PIJ
CSB-EP752299PIJ
CSB-BP752299PIJ
CSB-MP752299PIJ
CSB-EP752299PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S14 (rpsN) CSB-YP752300PIJ
CSB-EP752300PIJ
CSB-BP752300PIJ
CSB-MP752300PIJ
CSB-EP752300PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S11 (rpsK) CSB-YP752301PIJ
CSB-EP752301PIJ
CSB-BP752301PIJ
CSB-MP752301PIJ
CSB-EP752301PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Shikimate dehydrogenase (aroE) CSB-YP752302PIJ
CSB-EP752302PIJ
CSB-BP752302PIJ
CSB-MP752302PIJ
CSB-EP752302PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Porphobilinogen deaminase (hemC) CSB-YP752303PIJ
CSB-EP752303PIJ
CSB-BP752303PIJ
CSB-MP752303PIJ
CSB-EP752303PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Protein hfq (hfq) CSB-YP752304PIJ
CSB-EP752304PIJ
CSB-BP752304PIJ
CSB-MP752304PIJ
CSB-EP752304PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Arginine repressor (argR) CSB-YP752305PIJ
CSB-EP752305PIJ
CSB-BP752305PIJ
CSB-MP752305PIJ
CSB-EP752305PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribosome maturation factor RimP (rimP) CSB-YP752306PIJ
CSB-EP752306PIJ
CSB-BP752306PIJ
CSB-MP752306PIJ
CSB-EP752306PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Aspartate carbamoyltransferase (pyrB) CSB-YP752307PIJ
CSB-EP752307PIJ
CSB-BP752307PIJ
CSB-MP752307PIJ
CSB-EP752307PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Sec-independent protein translocase protein TatA (tatA), partial CSB-YP752308PIJ
CSB-EP752308PIJ
CSB-BP752308PIJ
CSB-MP752308PIJ
CSB-EP752308PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Glucose-6-phosphate isomerase (pgi), partial CSB-YP752309PIJ
CSB-EP752309PIJ
CSB-BP752309PIJ
CSB-MP752309PIJ
CSB-EP752309PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0271 protein plu4304 (plu4304) CSB-YP752310PIJ
CSB-EP752310PIJ
CSB-BP752310PIJ
CSB-MP752310PIJ
CSB-EP752310PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 3-dehydroquinate dehydratase (aroQ) CSB-YP752311PIJ
CSB-EP752311PIJ
CSB-BP752311PIJ
CSB-MP752311PIJ
CSB-EP752311PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii DnaA initiator-associating protein DiaA (diaA) CSB-YP752312PIJ
CSB-EP752312PIJ
CSB-BP752312PIJ
CSB-MP752312PIJ
CSB-EP752312PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Bifunctional protein HldE (hldE) CSB-YP752313PIJ
CSB-EP752313PIJ
CSB-BP752313PIJ
CSB-MP752313PIJ
CSB-EP752313PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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