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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Gloeobacter violaceus N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP752434GCI
CSB-EP752434GCI
CSB-BP752434GCI
CSB-MP752434GCI
CSB-EP752434GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Serine--tRNA ligase (serS) CSB-YP752435GCI
CSB-EP752435GCI
CSB-BP752435GCI
CSB-MP752435GCI
CSB-EP752435GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP752436GCI1
CSB-EP752436GCI1
CSB-BP752436GCI1
CSB-MP752436GCI1
CSB-EP752436GCI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Peptidyl-tRNA hydrolase (pth) CSB-YP752437GCI
CSB-EP752437GCI
CSB-BP752437GCI
CSB-MP752437GCI
CSB-EP752437GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Chromophore lyase CpcT/CpeT 1 (cpcT1) CSB-YP752438GCI
CSB-EP752438GCI
CSB-BP752438GCI
CSB-MP752438GCI
CSB-EP752438GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus tRNA (Ile)-lysidine synthase (tilS) CSB-YP752439GCI
CSB-EP752439GCI
CSB-BP752439GCI
CSB-MP752439GCI
CSB-EP752439GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus NAD (P)H-quinone oxidoreductase chain 4 (ndhD), partial CSB-YP752440GCI
CSB-EP752440GCI
CSB-BP752440GCI
CSB-MP752440GCI
CSB-EP752440GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP752441GCI
CSB-EP752441GCI
CSB-BP752441GCI
CSB-MP752441GCI
CSB-EP752441GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Glutamate 5-kinase (proB) CSB-YP752442GCI
CSB-EP752442GCI
CSB-BP752442GCI
CSB-MP752442GCI
CSB-EP752442GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Putative membrane protein insertion efficiency factor (CV_4405) CSB-YP752443CKA
CSB-EP752443CKA
CSB-BP752443CKA
CSB-MP752443CKA
CSB-EP752443CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP752444CKA
CSB-EP752444CKA
CSB-BP752444CKA
CSB-MP752444CKA
CSB-EP752444CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP752445CKA
CSB-EP752445CKA
CSB-BP752445CKA
CSB-MP752445CKA
CSB-EP752445CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0145 protein CV_4322 (CV_4322) CSB-YP752446CKA
CSB-EP752446CKA
CSB-BP752446CKA
CSB-MP752446CKA
CSB-EP752446CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum LPS-assembly protein lptD (lptD), partial CSB-YP752447CKA
CSB-EP752447CKA
CSB-BP752447CKA
CSB-MP752447CKA
CSB-EP752447CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP752448CKA
CSB-EP752448CKA
CSB-BP752448CKA
CSB-MP752448CKA
CSB-EP752448CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S10 (rpsJ) CSB-YP752449CKA
CSB-EP752449CKA
CSB-BP752449CKA
CSB-MP752449CKA
CSB-EP752449CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L14 (rplN) CSB-YP752450CKA
CSB-EP752450CKA
CSB-BP752450CKA
CSB-MP752450CKA
CSB-EP752450CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S5 (rpsE) CSB-YP752451CKA
CSB-EP752451CKA
CSB-BP752451CKA
CSB-MP752451CKA
CSB-EP752451CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L17 (rplQ) CSB-YP752452CKA
CSB-EP752452CKA
CSB-BP752452CKA
CSB-MP752452CKA
CSB-EP752452CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP752453CKA
CSB-EP752453CKA
CSB-BP752453CKA
CSB-MP752453CKA
CSB-EP752453CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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