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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP754077CQF
CSB-EP754077CQF
CSB-BP754077CQF
CSB-MP754077CQF
CSB-EP754077CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uridylate kinase (pyrH) CSB-YP754078CQF
CSB-EP754078CQF
CSB-BP754078CQF
CSB-MP754078CQF
CSB-EP754078CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP754079CQF
CSB-EP754079CQF
CSB-BP754079CQF
CSB-MP754079CQF
CSB-EP754079CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized RNA methyltransferase DIP1398 (DIP1398) CSB-YP754080CQF
CSB-EP754080CQF
CSB-BP754080CQF
CSB-MP754080CQF
CSB-EP754080CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P (efp) CSB-YP754081CQF
CSB-EP754081CQF
CSB-BP754081CQF
CSB-MP754081CQF
CSB-EP754081CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbamoyl-phosphate synthase small chain (carA) CSB-YP754082CQF
CSB-EP754082CQF
CSB-BP754082CQF
CSB-MP754082CQF
CSB-EP754082CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP754083CQF
CSB-EP754083CQF
CSB-BP754083CQF
CSB-MP754083CQF
CSB-EP754083CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sec-independent protein translocase protein TatA (tatA), partial CSB-YP754084CQF
CSB-EP754084CQF
CSB-BP754084CQF
CSB-MP754084CQF
CSB-EP754084CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP754085CQF
CSB-EP754085CQF
CSB-BP754085CQF
CSB-MP754085CQF
CSB-EP754085CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP754086CQF
CSB-EP754086CQF
CSB-BP754086CQF
CSB-MP754086CQF
CSB-EP754086CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroxy-acid dehydratase (ilvD), partial CSB-YP754087CQF
CSB-EP754087CQF
CSB-BP754087CQF
CSB-MP754087CQF
CSB-EP754087CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit delta (atpH) CSB-YP754088CQF
CSB-EP754088CQF
CSB-BP754088CQF
CSB-MP754088CQF
CSB-EP754088CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP754089CQF
CSB-EP754089CQF
CSB-BP754089CQF
CSB-MP754089CQF
CSB-EP754089CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP754090CQF
CSB-EP754090CQF
CSB-BP754090CQF
CSB-MP754090CQF
CSB-EP754090CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-6-phosphate isomerase (pgi), partial CSB-YP754091CQF
CSB-EP754091CQF
CSB-BP754091CQF
CSB-MP754091CQF
CSB-EP754091CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SsrA-binding protein (smpB) CSB-YP754092CQF
CSB-EP754092CQF
CSB-BP754092CQF
CSB-MP754092CQF
CSB-EP754092CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein FolD (folD) CSB-YP754093CQF
CSB-EP754093CQF
CSB-BP754093CQF
CSB-MP754093CQF
CSB-EP754093CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium diphtheriae 30S ribosomal protein S4 (rpsD) CSB-YP754094CQF
CSB-EP754094CQF
CSB-BP754094CQF
CSB-MP754094CQF
CSB-EP754094CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L6 (rplF) CSB-YP754095CQF
CSB-EP754095CQF
CSB-BP754095CQF
CSB-MP754095CQF
CSB-EP754095CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L29 (rpmC) CSB-YP754096CQF
CSB-EP754096CQF
CSB-BP754096CQF
CSB-MP754096CQF
CSB-EP754096CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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