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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L24 (rplX) CSB-YP753676BAAE
CSB-EP753676BAAE
CSB-BP753676BAAE
CSB-MP753676BAAE
CSB-EP753676BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L23 (rplW) CSB-YP753677BAAE
CSB-EP753677BAAE
CSB-BP753677BAAE
CSB-MP753677BAAE
CSB-EP753677BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Elongation factor G (fusA), partial CSB-YP753678BAAE
CSB-EP753678BAAE
CSB-BP753678BAAE
CSB-MP753678BAAE
CSB-EP753678BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Cysteine--tRNA ligase (cysS) CSB-YP753679BAAE
CSB-EP753679BAAE
CSB-BP753679BAAE
CSB-MP753679BAAE
CSB-EP753679BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian DNA integrity scanning protein DisA (disA) CSB-YP753680BAAE
CSB-EP753680BAAE
CSB-BP753680BAAE
CSB-MP753680BAAE
CSB-EP753680BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Thymidylate kinase (tmk) CSB-YP753681BAAE
CSB-EP753681BAAE
CSB-BP753681BAAE
CSB-MP753681BAAE
CSB-EP753681BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dephospho-CoA kinase (coaE) CSB-YP753682BQE
CSB-EP753682BQE
CSB-BP753682BQE
CSB-MP753682BQE
CSB-EP753682BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA replication and repair protein recF (recF) CSB-YP753683BQE
CSB-EP753683BQE
CSB-BP753683BQE
CSB-MP753683BQE
CSB-EP753683BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica BURP domain-containing protein 6 (BURP6) CSB-YP753684OFG
CSB-EP753684OFG
CSB-BP753684OFG
CSB-MP753684OFG
CSB-EP753684OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP753685IKB
CSB-EP753685IKB
CSB-BP753685IKB
CSB-MP753685IKB
CSB-EP753685IKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Isochorismatase domain-containing protein 1 (Isoc1) CSB-YP753686RA
CSB-EP753686RA
CSB-BP753686RA
CSB-MP753686RA
CSB-EP753686RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Glycine N-acyltransferase (GLYAT) CSB-YP753687HU
CSB-EP753687HU
CSB-BP753687HU
CSB-MP753687HU
CSB-EP753687HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sesquipedalian-2 (FAM109B) CSB-YP753688HU
CSB-BP753688HU
CSB-MP753688HU
CSB-EP753688HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable serine/threonine-protein kinase WNK11 (WNK11) CSB-YP753689DOA
CSB-EP753689DOA
CSB-BP753689DOA
CSB-MP753689DOA
CSB-EP753689DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Transmembrane protease serine 11B-like protein (Tmprss11bnl), partial CSB-YP753690RA
CSB-EP753690RA
CSB-BP753690RA
CSB-MP753690RA
CSB-EP753690RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ovostatin homolog 2 (OVOS2), partial CSB-YP753691HU
CSB-EP753691HU
CSB-BP753691HU
CSB-MP753691HU
CSB-EP753691HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serine protease inhibitor Kazal-type 6 (Spink6) CSB-YP753692RA
CSB-EP753692RA
CSB-BP753692RA
CSB-MP753692RA
CSB-EP753692RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Complement C1r subcomponent-like protein (C1rl) CSB-YP753693RA
CSB-BP753693RA
CSB-MP753693RA
CSB-EP753693RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Interferon alpha-inducible protein 6 (IFI6), partial CSB-YP753694BO1
CSB-EP753694BO1
CSB-BP753694BO1
CSB-MP753694BO1
CSB-EP753694BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Homeobox protein Hox-B2a (hoxb2a) CSB-YP753695TKY
CSB-EP753695TKY
CSB-BP753695TKY
CSB-MP753695TKY
CSB-EP753695TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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