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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus 2-isopropylmalate synthase (leuA), partial CSB-YP754879BQN
CSB-EP754879BQN
CSB-BP754879BQN
CSB-MP754879BQN
CSB-EP754879BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-alanine--poly (phosphoribitol) ligase subunit 1 (dltA), partial CSB-YP754880BQN
CSB-EP754880BQN
CSB-BP754880BQN
CSB-MP754880BQN
CSB-EP754880BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lactate utilization protein B (lutB) CSB-YP754881BQN
CSB-EP754881BQN
CSB-BP754881BQN
CSB-MP754881BQN
CSB-EP754881BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Indole-3-glycerol phosphate synthase (trpC) CSB-YP754882BQN
CSB-EP754882BQN
CSB-BP754882BQN
CSB-MP754882BQN
CSB-EP754882BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP754883BQN
CSB-EP754883BQN
CSB-BP754883BQN
CSB-MP754883BQN
CSB-EP754883BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0342 protein BCE_0953 (BCE_0953) CSB-YP754884BQN
CSB-EP754884BQN
CSB-BP754884BQN
CSB-MP754884BQN
CSB-EP754884BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Potassium-transporting ATPase C chain (kdpC), partial CSB-YP754885BQN1
CSB-EP754885BQN1
CSB-BP754885BQN1
CSB-MP754885BQN1
CSB-EP754885BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein K (sspK) CSB-YP754886BQN
CSB-EP754886BQN
CSB-BP754886BQN
CSB-MP754886BQN
CSB-EP754886BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Arginine deiminase (arcA) CSB-YP754887BQN
CSB-EP754887BQN
CSB-BP754887BQN
CSB-MP754887BQN
CSB-EP754887BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP754888BQN
CSB-EP754888BQN
CSB-BP754888BQN
CSB-MP754888BQN
CSB-EP754888BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP754889BQN
CSB-EP754889BQN
CSB-BP754889BQN
CSB-MP754889BQN
CSB-EP754889BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP754890BQN
CSB-EP754890BQN
CSB-BP754890BQN
CSB-MP754890BQN
CSB-EP754890BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP754891BQN
CSB-EP754891BQN
CSB-BP754891BQN
CSB-MP754891BQN
CSB-EP754891BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S11 (rpsK) CSB-YP754892BQN
CSB-EP754892BQN
CSB-BP754892BQN
CSB-MP754892BQN
CSB-EP754892BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S8 (rpsH) CSB-YP754893BQN
CSB-EP754893BQN
CSB-BP754893BQN
CSB-MP754893BQN
CSB-EP754893BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S17 (rpsQ) CSB-YP754894BQN
CSB-EP754894BQN
CSB-BP754894BQN
CSB-MP754894BQN
CSB-EP754894BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP754895BQN
CSB-EP754895BQN
CSB-BP754895BQN
CSB-MP754895BQN
CSB-EP754895BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP754896BQN
CSB-EP754896BQN
CSB-BP754896BQN
CSB-MP754896BQN
CSB-EP754896BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0213 protein BCE_0033 (BCE_0033) CSB-YP754897BQN
CSB-EP754897BQN
CSB-BP754897BQN
CSB-MP754897BQN
CSB-EP754897BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome maturation factor RimM (rimM) CSB-YP754898WAQ
CSB-EP754898WAQ
CSB-BP754898WAQ
CSB-MP754898WAQ
CSB-EP754898WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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