Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria monocytogenes serotype 4b Argininosuccinate synthase (argG) CSB-YP754679LAAD
CSB-EP754679LAAD
CSB-BP754679LAAD
CSB-MP754679LAAD
CSB-EP754679LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 10 kDa chaperonin (groS) CSB-YP754680LAAD
CSB-EP754680LAAD
CSB-BP754680LAAD
CSB-MP754680LAAD
CSB-EP754680LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L32-2 (rpmF2) CSB-YP754681LAAD
CSB-EP754681LAAD
CSB-BP754681LAAD
CSB-MP754681LAAD
CSB-EP754681LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Diaminopimelate epimerase (dapF) CSB-YP754682LAAD
CSB-EP754682LAAD
CSB-BP754682LAAD
CSB-MP754682LAAD
CSB-EP754682LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Ketol-acid reductoisomerase (ilvC) CSB-YP754683LAAD
CSB-EP754683LAAD
CSB-BP754683LAAD
CSB-MP754683LAAD
CSB-EP754683LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Phosphopentomutase (deoB) CSB-YP754684LAAD
CSB-EP754684LAAD
CSB-BP754684LAAD
CSB-MP754684LAAD
CSB-EP754684LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Demethylmenaquinone methyltransferase (ubiE) CSB-YP754685LAAD
CSB-EP754685LAAD
CSB-BP754685LAAD
CSB-MP754685LAAD
CSB-EP754685LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Dihydrodipicolinate reductase (dapB) CSB-YP754686LAAD
CSB-EP754686LAAD
CSB-BP754686LAAD
CSB-MP754686LAAD
CSB-EP754686LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Aspartate 1-decarboxylase (panD) CSB-YP754687LAAD
CSB-EP754687LAAD
CSB-BP754687LAAD
CSB-MP754687LAAD
CSB-EP754687LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Thymidylate synthase (thyA) CSB-YP754688LAAD
CSB-EP754688LAAD
CSB-BP754688LAAD
CSB-MP754688LAAD
CSB-EP754688LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Carbamoyl-phosphate synthase small chain (carA) CSB-YP754689LAAD
CSB-EP754689LAAD
CSB-BP754689LAAD
CSB-MP754689LAAD
CSB-EP754689LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP754690LAAD
CSB-EP754690LAAD
CSB-BP754690LAAD
CSB-MP754690LAAD
CSB-EP754690LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Phosphate acyltransferase (plsX) CSB-YP754691LAAD
CSB-EP754691LAAD
CSB-BP754691LAAD
CSB-MP754691LAAD
CSB-EP754691LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP754692LAAD
CSB-EP754692LAAD
CSB-BP754692LAAD
CSB-MP754692LAAD
CSB-EP754692LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP754693LAAD
CSB-EP754693LAAD
CSB-BP754693LAAD
CSB-MP754693LAAD
CSB-EP754693LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP754694LAAD
CSB-EP754694LAAD
CSB-BP754694LAAD
CSB-MP754694LAAD
CSB-EP754694LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Indole-3-glycerol phosphate synthase (trpC) CSB-YP754695LAAD
CSB-EP754695LAAD
CSB-BP754695LAAD
CSB-MP754695LAAD
CSB-EP754695LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP754696LAAD
CSB-EP754696LAAD
CSB-BP754696LAAD
CSB-MP754696LAAD
CSB-EP754696LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Acetylornithine aminotransferase (argD) CSB-YP754697LAAD
CSB-EP754697LAAD
CSB-BP754697LAAD
CSB-MP754697LAAD
CSB-EP754697LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0756 membrane protein LMOf2365_1590 (LMOf2365_1590), partial CSB-YP754698LAAD1
CSB-EP754698LAAD1
CSB-BP754698LAAD1
CSB-MP754698LAAD1
CSB-EP754698LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>