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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mouse Rho GTPase-activating protein 20 (Arhgap20), partial CSB-YP753696MO
CSB-EP753696MO
CSB-BP753696MO
CSB-MP753696MO
CSB-EP753696MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Keratin, type I cytoskeletal 42 (Krt42) CSB-YP753697MO
CSB-EP753697MO
CSB-BP753697MO
CSB-MP753697MO
CSB-EP753697MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Keratin, type II cytoskeletal 4 (Krt4) CSB-YP753698RA
CSB-EP753698RA
CSB-BP753698RA
CSB-MP753698RA
CSB-EP753698RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Keratin, type II cytoskeletal 1 (Krt1) CSB-YP753699RA
CSB-EP753699RA
CSB-BP753699RA
CSB-MP753699RA
CSB-EP753699RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Developmental pluripotency-associated protein 3 (Dppa3) CSB-YP753700RA
CSB-EP753700RA
CSB-BP753700RA
CSB-MP753700RA
CSB-EP753700RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat WAS/WASL-interacting protein family member 1 (Wipf1) CSB-YP753701RA
CSB-EP753701RA
CSB-BP753701RA
CSB-MP753701RA
CSB-EP753701RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Vesicular glutamate transporter 1 (slc17a7), partial CSB-YP753702XBE
CSB-EP753702XBE
CSB-BP753702XBE
CSB-MP753702XBE
CSB-EP753702XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histone-binding protein RBBP4-B (rbbp4-b) CSB-YP753703XBE
CSB-EP753703XBE
CSB-BP753703XBE
CSB-MP753703XBE
CSB-EP753703XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mediator of RNA polymerase II transcription subunit 1 (med1), partial CSB-YP753704XBE
CSB-EP753704XBE
CSB-BP753704XBE
CSB-MP753704XBE
CSB-EP753704XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis PDZ and LIM domain protein 7 (pdlim7) CSB-YP753705XBE
CSB-EP753705XBE
CSB-BP753705XBE
CSB-MP753705XBE
CSB-EP753705XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tetratricopeptide repeat protein 30A (ttc30a), partial CSB-YP753706XBE
CSB-EP753706XBE
CSB-BP753706XBE
CSB-MP753706XBE
CSB-EP753706XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein FAM100B-A (fam100b-a) CSB-YP753707XBE
CSB-EP753707XBE
CSB-BP753707XBE
CSB-MP753707XBE
CSB-EP753707XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human LYR motif-containing protein 5 (LYRM5) CSB-YP753708HU
CSB-EP753708HU
CSB-BP753708HU
CSB-MP753708HU
CSB-EP753708HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NADH-cytochrome b5 reductase-like (CYB5RL) CSB-YP753709HU
CSB-EP753709HU
CSB-BP753709HU
CSB-MP753709HU
CSB-EP753709HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ras-related protein Rab-12 (RAB12) CSB-BP753710HU
CSB-MP753710HU
CSB-EP753710HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tau-tubulin kinase 2 (TTBK2), partial CSB-YP753711HU
CSB-BP753711HU
CSB-MP753711HU
CSB-EP753711HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio RNA-binding protein 4.1 (rbm4.1) CSB-YP753712DIL
CSB-EP753712DIL
CSB-BP753712DIL
CSB-MP753712DIL
CSB-EP753712DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Complement C1q tumor necrosis factor-related protein 6 (C1qtnf6) CSB-BP753714MO
CSB-MP753714MO
CSB-EP753714MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein phosphatase 1 regulatory subunit 21 (ppp1r21), partial CSB-YP753715XBE
CSB-EP753715XBE
CSB-BP753715XBE
CSB-MP753715XBE
CSB-EP753715XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis CCR4-NOT transcription complex subunit 6-like-A (cnot6l-a), partial CSB-YP753716XBE
CSB-EP753716XBE
CSB-BP753716XBE
CSB-MP753716XBE
CSB-EP753716XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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