Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L22 (rplV) CSB-YP754097CQF
CSB-EP754097CQF
CSB-BP754097CQF
CSB-MP754097CQF
CSB-EP754097CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP754098CQF
CSB-EP754098CQF
CSB-BP754098CQF
CSB-MP754098CQF
CSB-EP754098CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0133 protein DIP0260 (DIP0260) CSB-YP754099CQF
CSB-EP754099CQF
CSB-BP754099CQF
CSB-MP754099CQF
CSB-EP754099CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable protein phosphatase 2C 65 (At5g01700) CSB-YP754100DOA
CSB-EP754100DOA
CSB-BP754100DOA
CSB-MP754100DOA
CSB-EP754100DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At2g26850 (At2g26850) CSB-YP754101DOA
CSB-EP754101DOA
CSB-BP754101DOA
CSB-MP754101DOA
CSB-EP754101DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF015 (ERF015) CSB-YP754102DOA
CSB-EP754102DOA
CSB-BP754102DOA
CSB-MP754102DOA
CSB-EP754102DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Monothiol glutaredoxin-S7 (GRXS7) CSB-YP754103DOA
CSB-EP754103DOA
CSB-BP754103DOA
CSB-MP754103DOA
CSB-EP754103DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Flowering time control protein FY (FY), partial CSB-YP754104DOA
CSB-EP754104DOA
CSB-BP754104DOA
CSB-MP754104DOA
CSB-EP754104DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ATL76 (ATL76), partial CSB-YP754105DOA1
CSB-EP754105DOA1
CSB-BP754105DOA1
CSB-MP754105DOA1
CSB-EP754105DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Defensin-like protein 95 (At4g22235) CSB-YP754106DOA
CSB-EP754106DOA
CSB-BP754106DOA
CSB-MP754106DOA
CSB-EP754106DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nudix hydrolase 10 (NUDT10) CSB-YP754107DOA
CSB-EP754107DOA
CSB-BP754107DOA
CSB-MP754107DOA
CSB-EP754107DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calmodulin-binding transcription activator 2 (CMTA2), partial CSB-YP754108DOA
CSB-EP754108DOA
CSB-BP754108DOA
CSB-MP754108DOA
CSB-EP754108DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g04790, mitochondrial (At4g04790), partial CSB-YP754109DOA
CSB-EP754109DOA
CSB-BP754109DOA
CSB-MP754109DOA
CSB-EP754109DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL78 (ATL78), partial CSB-YP754110DOA1
CSB-EP754110DOA1
CSB-BP754110DOA1
CSB-MP754110DOA1
CSB-EP754110DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein FAM73A (fam73a), partial CSB-YP754111XBE1
CSB-EP754111XBE1
CSB-BP754111XBE1
CSB-MP754111XBE1
CSB-EP754111XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase SH3RF1 (sh3rf1), partial CSB-YP754112XBE
CSB-EP754112XBE
CSB-BP754112XBE
CSB-MP754112XBE
CSB-EP754112XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase RNF8-B (rnf8-b), partial CSB-YP754113XBE
CSB-EP754113XBE
CSB-BP754113XBE
CSB-MP754113XBE
CSB-EP754113XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Malignant T-cell-amplified sequence 1-B (mcts1-b) CSB-YP754114XBE
CSB-EP754114XBE
CSB-BP754114XBE
CSB-MP754114XBE
CSB-EP754114XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Golgi to ER traffic protein 4 homolog A (get4-a) CSB-YP754115XBE
CSB-EP754115XBE
CSB-BP754115XBE
CSB-MP754115XBE
CSB-EP754115XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Zinc finger protein Pegasus (ikzf5) CSB-YP754116XBE
CSB-EP754116XBE
CSB-BP754116XBE
CSB-MP754116XBE
CSB-EP754116XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>