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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photobacterium profundum LexA repressor (lexA) CSB-YP753897PIG
CSB-EP753897PIG
CSB-BP753897PIG
CSB-MP753897PIG
CSB-EP753897PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Phosphomethylpyrimidine synthase (thiC), partial CSB-YP753898PIG
CSB-EP753898PIG
CSB-BP753898PIG
CSB-MP753898PIG
CSB-EP753898PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 3-ketoacyl-CoA thiolase (fadA) CSB-YP753899PIG
CSB-EP753899PIG
CSB-BP753899PIG
CSB-MP753899PIG
CSB-EP753899PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 2-amino-5-formylamino-6-ribosylaminopyrimidin-4 (3H)-one 5'-monophosphate deformylase (arfB) CSB-YP753900MSB
CSB-EP753900MSB
CSB-BP753900MSB
CSB-MP753900MSB
CSB-EP753900MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Tungsten-containing formylmethanofuran dehydrogenase 2 subunit B (fwdB) CSB-YP753901MSB
CSB-EP753901MSB
CSB-BP753901MSB
CSB-MP753901MSB
CSB-EP753901MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0179 protein MMP1679 (MMP1679) CSB-YP753902MSB
CSB-EP753902MSB
CSB-BP753902MSB
CSB-MP753902MSB
CSB-EP753902MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Tetrahydromethanopterin S-methyltransferase subunit E (mtrE), partial CSB-YP753903MSB1
CSB-EP753903MSB1
CSB-BP753903MSB1
CSB-MP753903MSB1
CSB-EP753903MSB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Alanine racemase (alr) CSB-YP753904MSB
CSB-EP753904MSB
CSB-BP753904MSB
CSB-MP753904MSB
CSB-EP753904MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable inorganic polyphosphate/ATP-NAD kinase (ppnK), partial CSB-YP753905MSB
CSB-EP753905MSB
CSB-BP753905MSB
CSB-MP753905MSB
CSB-EP753905MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0348 protein MMP1471 (MMP1471) CSB-YP753906MSB
CSB-EP753906MSB
CSB-BP753906MSB
CSB-MP753906MSB
CSB-EP753906MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0210 protein MMP1427 (MMP1427) CSB-YP753907MSB
CSB-EP753907MSB
CSB-BP753907MSB
CSB-MP753907MSB
CSB-EP753907MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S5P (rps5p) CSB-YP753908MSB
CSB-EP753908MSB
CSB-BP753908MSB
CSB-MP753908MSB
CSB-EP753908MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L29P (rpl29p) CSB-YP753909MSB
CSB-EP753909MSB
CSB-BP753909MSB
CSB-MP753909MSB
CSB-EP753909MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Elongation factor 1-beta (ef1b) CSB-YP753910MSB
CSB-EP753910MSB
CSB-BP753910MSB
CSB-MP753910MSB
CSB-EP753910MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0095 protein MMP1286 (MMP1286) CSB-YP753911MSB
CSB-EP753911MSB
CSB-BP753911MSB
CSB-MP753911MSB
CSB-EP753911MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamyl-tRNA (Gln) amidotransferase subunit E (gatE), partial CSB-YP753912MSB
CSB-EP753912MSB
CSB-BP753912MSB
CSB-MP753912MSB
CSB-EP753912MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 2 subunit gamma (eif2g) CSB-YP753913MSB
CSB-EP753913MSB
CSB-BP753913MSB
CSB-MP753913MSB
CSB-EP753913MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribose-5-phosphate isomerase A (rpiA) CSB-YP753914MSB
CSB-EP753914MSB
CSB-BP753914MSB
CSB-MP753914MSB
CSB-EP753914MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Signal recognition particle 19 kDa protein (srp19) CSB-YP753915MSB
CSB-EP753915MSB
CSB-BP753915MSB
CSB-MP753915MSB
CSB-EP753915MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Anthranilate phosphoribosyltransferase (trpD) CSB-YP753916MSB
CSB-EP753916MSB
CSB-BP753916MSB
CSB-MP753916MSB
CSB-EP753916MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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