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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Photobacterium profundum 50S ribosomal protein L1 (rplA) CSB-YP753837PIG
CSB-EP753837PIG
CSB-BP753837PIG
CSB-MP753837PIG
CSB-EP753837PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP753838PIG
CSB-EP753838PIG
CSB-BP753838PIG
CSB-MP753838PIG
CSB-EP753838PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribosomal protein L11 methyltransferase (prmA) CSB-YP753839PIG
CSB-EP753839PIG
CSB-BP753839PIG
CSB-MP753839PIG
CSB-EP753839PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Fumarate reductase subunit D (frdD), partial CSB-YP753840PIG1
CSB-EP753840PIG1
CSB-BP753840PIG1
CSB-MP753840PIG1
CSB-EP753840PIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum HTH-type transcriptional repressor NsrR (nsrR) CSB-YP753841PIG
CSB-EP753841PIG
CSB-BP753841PIG
CSB-MP753841PIG
CSB-EP753841PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Lipid II flippase FtsW (ftsW), partial CSB-YP753842PIG
CSB-EP753842PIG
CSB-BP753842PIG
CSB-MP753842PIG
CSB-EP753842PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Dephospho-CoA kinase (coaE) CSB-YP753843PIG
CSB-EP753843PIG
CSB-BP753843PIG
CSB-MP753843PIG
CSB-EP753843PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP753844PIG
CSB-EP753844PIG
CSB-BP753844PIG
CSB-MP753844PIG
CSB-EP753844PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UPF0301 protein PBPRA3139 (PBPRA3139) CSB-YP753845PIG
CSB-EP753845PIG
CSB-BP753845PIG
CSB-MP753845PIG
CSB-EP753845PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribose-5-phosphate isomerase A (rpiA) CSB-YP753846PIG
CSB-EP753846PIG
CSB-BP753846PIG
CSB-MP753846PIG
CSB-EP753846PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP753847PIG
CSB-EP753847PIG
CSB-BP753847PIG
CSB-MP753847PIG
CSB-EP753847PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Alanine--tRNA ligase (alaS), partial CSB-YP753848PIG
CSB-EP753848PIG
CSB-BP753848PIG
CSB-MP753848PIG
CSB-EP753848PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Protein syd (syd) CSB-YP753849PIG
CSB-EP753849PIG
CSB-BP753849PIG
CSB-MP753849PIG
CSB-EP753849PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Uridylate kinase (pyrH) CSB-YP753850PIG
CSB-EP753850PIG
CSB-BP753850PIG
CSB-MP753850PIG
CSB-EP753850PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Proline--tRNA ligase (proS), partial CSB-YP753851PIG
CSB-EP753851PIG
CSB-BP753851PIG
CSB-MP753851PIG
CSB-EP753851PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Leucine--tRNA ligase (leuS), partial CSB-YP753852PIG
CSB-EP753852PIG
CSB-BP753852PIG
CSB-MP753852PIG
CSB-EP753852PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP753853PIG
CSB-EP753853PIG
CSB-BP753853PIG
CSB-MP753853PIG
CSB-EP753853PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Chorismate synthase (aroC) CSB-YP753854PIG
CSB-EP753854PIG
CSB-BP753854PIG
CSB-MP753854PIG
CSB-EP753854PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP753855PIG
CSB-EP753855PIG
CSB-BP753855PIG
CSB-MP753855PIG
CSB-EP753855PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum tRNA 2-selenouridine synthase (selU) CSB-YP753856PIG
CSB-EP753856PIG
CSB-BP753856PIG
CSB-MP753856PIG
CSB-EP753856PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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