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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Homoserine O-acetyltransferase (metX) CSB-YP754819LEV
CSB-EP754819LEV
CSB-BP754819LEV
CSB-MP754819LEV
CSB-EP754819LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Phosphoheptose isomerase (gmhA) CSB-YP754820LEV
CSB-EP754820LEV
CSB-BP754820LEV
CSB-MP754820LEV
CSB-EP754820LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Biotin synthase (bioB) CSB-YP754821LEV
CSB-EP754821LEV
CSB-BP754821LEV
CSB-MP754821LEV
CSB-EP754821LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP754822LEV
CSB-EP754822LEV
CSB-BP754822LEV
CSB-MP754822LEV
CSB-EP754822LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Tyrosine recombinase XerC (xerC) CSB-YP754823LEV
CSB-EP754823LEV
CSB-BP754823LEV
CSB-MP754823LEV
CSB-EP754823LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Vitamin B12-dependent ribonucleotide reductase (nrdJ), partial CSB-YP754824LEV
CSB-EP754824LEV
CSB-BP754824LEV
CSB-MP754824LEV
CSB-EP754824LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Ribosome maturation factor RimM (rimM) CSB-YP754825LEV
CSB-EP754825LEV
CSB-BP754825LEV
CSB-MP754825LEV
CSB-EP754825LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP754826LEV
CSB-EP754826LEV
CSB-BP754826LEV
CSB-MP754826LEV
CSB-EP754826LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni ATP synthase subunit beta (atpD) CSB-YP754827LEV
CSB-EP754827LEV
CSB-BP754827LEV
CSB-MP754827LEV
CSB-EP754827LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Uncharacterized RNA methyltransferase LIC_11085 (LIC_11085) CSB-YP754828LEV
CSB-EP754828LEV
CSB-BP754828LEV
CSB-MP754828LEV
CSB-EP754828LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Glutamate racemase (murI) CSB-YP754829LEV
CSB-EP754829LEV
CSB-BP754829LEV
CSB-MP754829LEV
CSB-EP754829LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 30S ribosomal protein S7 (rpsG) CSB-YP754830LEV
CSB-EP754830LEV
CSB-BP754830LEV
CSB-MP754830LEV
CSB-EP754830LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP754831LEV
CSB-EP754831LEV
CSB-BP754831LEV
CSB-MP754831LEV
CSB-EP754831LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Probable cytosol aminopeptidase (pepA) CSB-YP754832LEV
CSB-EP754832LEV
CSB-BP754832LEV
CSB-MP754832LEV
CSB-EP754832LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP754833LEV
CSB-EP754833LEV
CSB-BP754833LEV
CSB-MP754833LEV
CSB-EP754833LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Valine--tRNA ligase (valS), partial CSB-YP754834LEV
CSB-EP754834LEV
CSB-BP754834LEV
CSB-MP754834LEV
CSB-EP754834LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP754835LEV
CSB-EP754835LEV
CSB-BP754835LEV
CSB-MP754835LEV
CSB-EP754835LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP754836LEV
CSB-EP754836LEV
CSB-BP754836LEV
CSB-MP754836LEV
CSB-EP754836LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Membrane protein insertase YidC (yidC), partial CSB-YP754837LEV1
CSB-EP754837LEV1
CSB-BP754837LEV1
CSB-MP754837LEV1
CSB-EP754837LEV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Deoxycytidine triphosphate deaminase (dcd) CSB-YP754838LEV
CSB-EP754838LEV
CSB-BP754838LEV
CSB-MP754838LEV
CSB-EP754838LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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