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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter sulfurreducens Dihydroxy-acid dehydratase (ilvD), partial CSB-YP755019GBK
CSB-EP755019GBK
CSB-BP755019GBK
CSB-MP755019GBK
CSB-EP755019GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UPF0042 nucleotide-binding protein GSU1884 (GSU1884) CSB-YP755020GBK
CSB-EP755020GBK
CSB-BP755020GBK
CSB-MP755020GBK
CSB-EP755020GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP755021GBK
CSB-EP755021GBK
CSB-BP755021GBK
CSB-MP755021GBK
CSB-EP755021GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 1-deoxy-D-xylulose-5-phosphate synthase 2 (dxs2), partial CSB-YP755022GBK
CSB-EP755022GBK
CSB-BP755022GBK
CSB-MP755022GBK
CSB-EP755022GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP755023GBK
CSB-EP755023GBK
CSB-BP755023GBK
CSB-MP755023GBK
CSB-EP755023GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP755024GBK
CSB-EP755024GBK
CSB-BP755024GBK
CSB-MP755024GBK
CSB-EP755024GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Threonine--tRNA ligase (thrS), partial CSB-YP755025GBK
CSB-EP755025GBK
CSB-BP755025GBK
CSB-MP755025GBK
CSB-EP755025GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP755026GBK
CSB-EP755026GBK
CSB-BP755026GBK
CSB-MP755026GBK
CSB-EP755026GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Deoxyguanosinetriphosphate triphosphohydrolase-like protein (GSU1246) CSB-YP755027GBK
CSB-EP755027GBK
CSB-BP755027GBK
CSB-MP755027GBK
CSB-EP755027GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP755028GBK
CSB-EP755028GBK
CSB-BP755028GBK
CSB-MP755028GBK
CSB-EP755028GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Uncharacterized transporter GSU1016 (GSU1016), partial CSB-YP755029GBK
CSB-EP755029GBK
CSB-BP755029GBK
CSB-MP755029GBK
CSB-EP755029GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP755030GBK
CSB-EP755030GBK
CSB-BP755030GBK
CSB-MP755030GBK
CSB-EP755030GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S6 (rpsF) CSB-YP755031GBK
CSB-EP755031GBK
CSB-BP755031GBK
CSB-MP755031GBK
CSB-EP755031GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L19 (rplS) CSB-YP755032GBK
CSB-EP755032GBK
CSB-BP755032GBK
CSB-MP755032GBK
CSB-EP755032GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP755033GBK
CSB-EP755033GBK
CSB-BP755033GBK
CSB-MP755033GBK
CSB-EP755033GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Elongation factor P 1 (efp1) CSB-YP755034GBK
CSB-EP755034GBK
CSB-BP755034GBK
CSB-MP755034GBK
CSB-EP755034GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP755035GBK
CSB-EP755035GBK
CSB-BP755035GBK
CSB-MP755035GBK
CSB-EP755035GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Chromosomal replication initiator protein DnaA (dnaA) CSB-YP755036GBK
CSB-EP755036GBK
CSB-BP755036GBK
CSB-MP755036GBK
CSB-EP755036GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Dihydrodipicolinate reductase (dapB) CSB-YP755037GBK
CSB-EP755037GBK
CSB-BP755037GBK
CSB-MP755037GBK
CSB-EP755037GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Methionyl-tRNA formyltransferase (fmt) CSB-YP755038GBK
CSB-EP755038GBK
CSB-BP755038GBK
CSB-MP755038GBK
CSB-EP755038GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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