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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP754839BQN
CSB-EP754839BQN
CSB-BP754839BQN
CSB-MP754839BQN
CSB-EP754839BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 (murA2) CSB-YP754840BQN
CSB-EP754840BQN
CSB-BP754840BQN
CSB-MP754840BQN
CSB-EP754840BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase subunit b (atpF), partial CSB-YP754841BQN
CSB-EP754841BQN
CSB-BP754841BQN
CSB-MP754841BQN
CSB-EP754841BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase subunit beta (atpD) CSB-YP754842BQN
CSB-EP754842BQN
CSB-BP754842BQN
CSB-MP754842BQN
CSB-EP754842BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 (murA1) CSB-YP754843BQN
CSB-EP754843BQN
CSB-BP754843BQN
CSB-MP754843BQN
CSB-EP754843BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UvrABC system protein B (uvrB), partial CSB-YP754844BQN
CSB-EP754844BQN
CSB-BP754844BQN
CSB-MP754844BQN
CSB-EP754844BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus SsrA-binding protein (smpB) CSB-YP754845BQN
CSB-EP754845BQN
CSB-BP754845BQN
CSB-MP754845BQN
CSB-EP754845BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lipoyl synthase (lipA) CSB-YP754846BQN
CSB-EP754846BQN
CSB-BP754846BQN
CSB-MP754846BQN
CSB-EP754846BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycogen synthase (glgA) CSB-YP754847BQN
CSB-EP754847BQN
CSB-BP754847BQN
CSB-MP754847BQN
CSB-EP754847BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable molybdenum cofactor guanylyltransferase (mobA) CSB-YP754848BQN
CSB-EP754848BQN
CSB-BP754848BQN
CSB-MP754848BQN
CSB-EP754848BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S4 (rpsD) CSB-YP754849BQN
CSB-EP754849BQN
CSB-BP754849BQN
CSB-MP754849BQN
CSB-EP754849BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP754850BQN
CSB-EP754850BQN
CSB-BP754850BQN
CSB-MP754850BQN
CSB-EP754850BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dephospho-CoA kinase (coaE) CSB-YP754851BQN
CSB-EP754851BQN
CSB-BP754851BQN
CSB-MP754851BQN
CSB-EP754851BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP754852BQN
CSB-EP754852BQN
CSB-BP754852BQN
CSB-MP754852BQN
CSB-EP754852BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UvrABC system protein C (uvrC), partial CSB-YP754853BQN
CSB-EP754853BQN
CSB-BP754853BQN
CSB-MP754853BQN
CSB-EP754853BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Valine--tRNA ligase (valS), partial CSB-YP754854BQN
CSB-EP754854BQN
CSB-BP754854BQN
CSB-MP754854BQN
CSB-EP754854BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP754855BQN
CSB-EP754855BQN
CSB-BP754855BQN
CSB-MP754855BQN
CSB-EP754855BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L33 3 (rpmG3) CSB-YP754856BQN
CSB-EP754856BQN
CSB-BP754856BQN
CSB-MP754856BQN
CSB-EP754856BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S21 (rpsU) CSB-YP754857BQN
CSB-EP754857BQN
CSB-BP754857BQN
CSB-MP754857BQN
CSB-EP754857BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N utilization substance protein B homolog (nusB) CSB-YP754858BQN
CSB-EP754858BQN
CSB-BP754858BQN
CSB-MP754858BQN
CSB-EP754858BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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