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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bordetella pertussis Histidinol-phosphate aminotransferase 2 (hisC2) CSB-YP756443BUA
CSB-EP756443BUA
CSB-BP756443BUA
CSB-MP756443BUA
CSB-EP756443BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Deoxyguanosinetriphosphate triphosphohydrolase-like protein (BP3658) CSB-YP756444BUA
CSB-EP756444BUA
CSB-BP756444BUA
CSB-MP756444BUA
CSB-EP756444BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 30S ribosomal protein S13 (rpsM) CSB-YP756445BUA
CSB-EP756445BUA
CSB-BP756445BUA
CSB-MP756445BUA
CSB-EP756445BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 30S ribosomal protein S8 (rpsH) CSB-YP756446BUA
CSB-EP756446BUA
CSB-BP756446BUA
CSB-MP756446BUA
CSB-EP756446BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 50S ribosomal protein L16 (rplP) CSB-YP756447BUA
CSB-EP756447BUA
CSB-BP756447BUA
CSB-MP756447BUA
CSB-EP756447BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Elongation factor G (fusA), partial CSB-YP756448BUA
CSB-EP756448BUA
CSB-BP756448BUA
CSB-MP756448BUA
CSB-EP756448BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Putative arginyl-tRNA--protein transferase (ate) CSB-YP756449BUA
CSB-EP756449BUA
CSB-BP756449BUA
CSB-MP756449BUA
CSB-EP756449BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP756450BUA
CSB-EP756450BUA
CSB-BP756450BUA
CSB-MP756450BUA
CSB-EP756450BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis ADP-dependent (S)-NAD (P)H-hydrate dehydratase (nnrD) CSB-YP756451BUA
CSB-EP756451BUA
CSB-BP756451BUA
CSB-MP756451BUA
CSB-EP756451BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis ATP synthase subunit a (atpB), partial CSB-YP756452BUA1
CSB-EP756452BUA1
CSB-BP756452BUA1
CSB-MP756452BUA1
CSB-EP756452BUA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Glutamine--tRNA ligase (glnS), partial CSB-YP756453BUA
CSB-EP756453BUA
CSB-BP756453BUA
CSB-MP756453BUA
CSB-EP756453BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP756454BUA
CSB-EP756454BUA
CSB-BP756454BUA
CSB-MP756454BUA
CSB-EP756454BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Molybdenum import ATP-binding protein ModC (modC) CSB-YP756455BUA
CSB-EP756455BUA
CSB-BP756455BUA
CSB-MP756455BUA
CSB-EP756455BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP756456BUA
CSB-EP756456BUA
CSB-BP756456BUA
CSB-MP756456BUA
CSB-EP756456BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Cell division protein FtsQ (ftsQ), partial CSB-YP756457BUA
CSB-EP756457BUA
CSB-BP756457BUA
CSB-MP756457BUA
CSB-EP756457BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 50S ribosomal protein L13 (rplM) CSB-YP756458BUA
CSB-EP756458BUA
CSB-BP756458BUA
CSB-MP756458BUA
CSB-EP756458BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis UPF0276 protein BP2925 (BP2925) CSB-YP756459BUA
CSB-EP756459BUA
CSB-BP756459BUA
CSB-MP756459BUA
CSB-EP756459BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 30S ribosomal protein S6 (rpsF) CSB-YP756460BUA
CSB-EP756460BUA
CSB-BP756460BUA
CSB-MP756460BUA
CSB-EP756460BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP756461BUA
CSB-EP756461BUA
CSB-BP756461BUA
CSB-MP756461BUA
CSB-EP756461BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 7-carboxy-7-deazaguanine synthase (queE) CSB-YP756462BUA
CSB-EP756462BUA
CSB-BP756462BUA
CSB-MP756462BUA
CSB-EP756462BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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