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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Haemophilus ducreyi ATP synthase subunit alpha (atpA), partial CSB-YP756403HSY
CSB-EP756403HSY
CSB-BP756403HSY
CSB-MP756403HSY
CSB-EP756403HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP756404BHM
CSB-EP756404BHM
CSB-BP756404BHM
CSB-MP756404BHM
CSB-EP756404BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S11 (rpsK) CSB-YP756405BHM
CSB-EP756405BHM
CSB-BP756405BHM
CSB-MP756405BHM
CSB-EP756405BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L24 (rplX) CSB-YP756406BHM
CSB-EP756406BHM
CSB-BP756406BHM
CSB-MP756406BHM
CSB-EP756406BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L23 (rplW) CSB-YP756407BHM
CSB-EP756407BHM
CSB-BP756407BHM
CSB-MP756407BHM
CSB-EP756407BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S16 (rpsP) CSB-YP756408BHM
CSB-EP756408BHM
CSB-BP756408BHM
CSB-MP756408BHM
CSB-EP756408BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), partial CSB-YP756409BHM
CSB-EP756409BHM
CSB-BP756409BHM
CSB-MP756409BHM
CSB-EP756409BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP756410BHM
CSB-EP756410BHM
CSB-BP756410BHM
CSB-MP756410BHM
CSB-EP756410BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isoleucine--tRNA ligase (ileS), partial CSB-YP756411BHM
CSB-EP756411BHM
CSB-BP756411BHM
CSB-MP756411BHM
CSB-EP756411BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-2 (infB), partial CSB-YP756412BHM
CSB-EP756412BHM
CSB-BP756412BHM
CSB-MP756412BHM
CSB-EP756412BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S18 (rpsR) CSB-YP756413BHM
CSB-EP756413BHM
CSB-BP756413BHM
CSB-MP756413BHM
CSB-EP756413BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP756414BHM1
CSB-EP756414BHM1
CSB-BP756414BHM1
CSB-MP756414BHM1
CSB-EP756414BHM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit beta (atpD) CSB-YP756415BHM
CSB-EP756415BHM
CSB-BP756415BHM
CSB-MP756415BHM
CSB-EP756415BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP756416BHM
CSB-EP756416BHM
CSB-BP756416BHM
CSB-MP756416BHM
CSB-EP756416BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine--tRNA ligase (argS), partial CSB-YP756417BHM
CSB-EP756417BHM
CSB-BP756417BHM
CSB-MP756417BHM
CSB-EP756417BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidine kinase (tdk) CSB-YP756418BHM
CSB-EP756418BHM
CSB-BP756418BHM
CSB-MP756418BHM
CSB-EP756418BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP756419BHM
CSB-EP756419BHM
CSB-BP756419BHM
CSB-MP756419BHM
CSB-EP756419BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP756420BHM
CSB-EP756420BHM
CSB-BP756420BHM
CSB-MP756420BHM
CSB-EP756420BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulator slyA (slyA) CSB-YP756421BHM
CSB-EP756421BHM
CSB-BP756421BHM
CSB-MP756421BHM
CSB-EP756421BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose-phosphate pyrophosphokinase (prs) CSB-YP756422BHM
CSB-EP756422BHM
CSB-BP756422BHM
CSB-MP756422BHM
CSB-EP756422BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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