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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acanthamoeba polyphaga mimivirus Probable DNA polymerase family X (MIMI_L318) CSB-YP756003ADAZ
CSB-EP756003ADAZ
CSB-BP756003ADAZ
CSB-MP756003ADAZ
CSB-EP756003ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Nucleoprotein (N) CSB-YP756004RID
CSB-EP756004RID
CSB-BP756004RID
CSB-MP756004RID
CSB-EP756004RID-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryphonectria parasitica mycoreovirus 1 Outer capsid protein VP3 (S3), partial CSB-YP756005CFAT
CSB-EP756005CFAT
CSB-BP756005CFAT
CSB-MP756005CFAT
CSB-EP756005CFAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human SARS coronavirus Protein non-structural 7b (7b), partial CSB-YP756006HQE
CSB-BP756006HQE
CSB-MP756006HQE
CSB-EP756006HQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Zinc finger CCCH domain-containing protein 14 (Zc3h14) CSB-YP756007RA
CSB-EP756007RA
CSB-BP756007RA
CSB-MP756007RA
CSB-EP756007RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse E3 ubiquitin-protein ligase UHRF2 (Uhrf2), partial CSB-YP756008MO
CSB-EP756008MO
CSB-BP756008MO
CSB-MP756008MO
CSB-EP756008MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse WD repeat-containing protein 91 (Wdr91) CSB-YP756009MO
CSB-EP756009MO
CSB-BP756009MO
CSB-MP756009MO
CSB-EP756009MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Importin-8 (Ipo8), partial CSB-YP756010MO
CSB-EP756010MO
CSB-BP756010MO
CSB-MP756010MO
CSB-EP756010MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Stromal membrane-associated protein 2 (Smap2) CSB-YP756011MO
CSB-EP756011MO
CSB-BP756011MO
CSB-MP756011MO
CSB-EP756011MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Mas-related G-protein coupled receptor member B5 (Mrgprb5), partial CSB-YP756012RA1
CSB-EP756012RA1
CSB-BP756012RA1
CSB-MP756012RA1
CSB-EP756012RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Retrotransposon-derived protein PEG10 (Peg10), partial CSB-YP756013MO
CSB-EP756013MO
CSB-BP756013MO
CSB-MP756013MO
CSB-EP756013MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Troponin T, slow skeletal muscle (Tnnt1) CSB-YP756014RA
CSB-EP756014RA
CSB-BP756014RA
CSB-MP756014RA
CSB-EP756014RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein FAM117A (Fam117a) CSB-YP756015MO
CSB-EP756015MO
CSB-BP756015MO
CSB-MP756015MO
CSB-EP756015MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Interleukin-22 receptor subunit alpha-2 (Il22ra2) CSB-YP756016RA
CSB-EP756016RA
CSB-BP756016RA
CSB-MP756016RA
CSB-EP756016RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ammonium transporter Rh type A (Rhag), partial CSB-YP756017RA
CSB-EP756017RA
CSB-BP756017RA
CSB-MP756017RA
CSB-EP756017RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Immunoglobulin superfamily member 21 (Igsf21) CSB-YP756018MO
CSB-EP756018MO
CSB-BP756018MO
CSB-MP756018MO
CSB-EP756018MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Splicing factor U2AF 26 kDa subunit (U2af1l4) CSB-YP756019RA
CSB-EP756019RA
CSB-BP756019RA
CSB-MP756019RA
CSB-EP756019RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ankyrin repeat and LEM domain-containing protein 2 (Ankle2), partial CSB-YP756020RA
CSB-EP756020RA
CSB-BP756020RA
CSB-MP756020RA
CSB-EP756020RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse F-box only protein 11 (Fbxo11), partial CSB-YP756021MO
CSB-EP756021MO
CSB-BP756021MO
CSB-MP756021MO
CSB-EP756021MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Small cell adhesion glycoprotein (Smagp), partial CSB-YP756022RA
CSB-EP756022RA
CSB-BP756022RA
CSB-MP756022RA
CSB-EP756022RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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