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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus subsp. pastoris Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP756203EYQ
CSB-EP756203EYQ
CSB-BP756203EYQ
CSB-MP756203EYQ
CSB-EP756203EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Threonine--tRNA ligase (thrS), partial CSB-YP756204EYQ
CSB-EP756204EYQ
CSB-BP756204EYQ
CSB-MP756204EYQ
CSB-EP756204EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP756205EYQ
CSB-EP756205EYQ
CSB-BP756205EYQ
CSB-MP756205EYQ
CSB-EP756205EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Uridylate kinase (pyrH) CSB-YP756206EYQ
CSB-EP756206EYQ
CSB-BP756206EYQ
CSB-MP756206EYQ
CSB-EP756206EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris UPF0234 protein PMM0481 (PMM0481) CSB-YP756207EYQ
CSB-EP756207EYQ
CSB-BP756207EYQ
CSB-MP756207EYQ
CSB-EP756207EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Apocytochrome f (petA), partial CSB-YP756208EYQ1
CSB-EP756208EYQ1
CSB-BP756208EYQ1
CSB-MP756208EYQ1
CSB-EP756208EYQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Probable malate:quinone oxidoreductase (mqo) CSB-YP756209EYQ
CSB-EP756209EYQ
CSB-BP756209EYQ
CSB-MP756209EYQ
CSB-EP756209EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 3-dehydroquinate dehydratase (aroQ) CSB-YP756210EYQ
CSB-EP756210EYQ
CSB-BP756210EYQ
CSB-MP756210EYQ
CSB-EP756210EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Transcriptional repressor NrdR (nrdR) CSB-YP756211EYQ
CSB-EP756211EYQ
CSB-BP756211EYQ
CSB-MP756211EYQ
CSB-EP756211EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris NAD (P)H-quinone oxidoreductase subunit K (ndhK) CSB-YP756212EYQ
CSB-EP756212EYQ
CSB-BP756212EYQ
CSB-MP756212EYQ
CSB-EP756212EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP756213EYQ
CSB-EP756213EYQ
CSB-BP756213EYQ
CSB-MP756213EYQ
CSB-EP756213EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP756214EYQ
CSB-EP756214EYQ
CSB-BP756214EYQ
CSB-MP756214EYQ
CSB-EP756214EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L7/L12 (rplL) CSB-YP756215EYQ
CSB-EP756215EYQ
CSB-BP756215EYQ
CSB-MP756215EYQ
CSB-EP756215EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris NAD (P)H-quinone oxidoreductase subunit H (ndhH) CSB-YP756216EYQ
CSB-EP756216EYQ
CSB-BP756216EYQ
CSB-MP756216EYQ
CSB-EP756216EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Photosystem I assembly protein ycf3 (ycf3) CSB-YP756217EYQ
CSB-EP756217EYQ
CSB-BP756217EYQ
CSB-MP756217EYQ
CSB-EP756217EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Photosystem II reaction center X protein (psbX), partial CSB-YP756218EYQ1
CSB-EP756218EYQ1
CSB-BP756218EYQ1
CSB-MP756218EYQ1
CSB-EP756218EYQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP756219EYQ
CSB-EP756219EYQ
CSB-BP756219EYQ
CSB-MP756219EYQ
CSB-EP756219EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Chaperone protein dnaK2 (dnaK2), partial CSB-YP756220EYO
CSB-EP756220EYO
CSB-BP756220EYO
CSB-MP756220EYO
CSB-EP756220EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Aspartate--tRNA ligase (aspS), partial CSB-YP756221EYO
CSB-EP756221EYO
CSB-BP756221EYO
CSB-MP756221EYO
CSB-EP756221EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Alanine--tRNA ligase (alaS), partial CSB-YP756222EYO
CSB-EP756222EYO
CSB-BP756222EYO
CSB-MP756222EYO
CSB-EP756222EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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