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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neurospora crassa Probable tRNA-splicing endonuclease subunit sen-34 (sen-34) CSB-YP755943NHA
CSB-EP755943NHA
CSB-BP755943NHA
CSB-MP755943NHA
CSB-EP755943NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Actin cytoskeleton-regulatory complex protein pan-1 (pan-1), partial CSB-YP755944NHA
CSB-EP755944NHA
CSB-BP755944NHA
CSB-MP755944NHA
CSB-EP755944NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Histone-lysine N-methyltransferase, H3 lysine-79 specific (dot-1), partial CSB-YP755945NHA
CSB-EP755945NHA
CSB-BP755945NHA
CSB-MP755945NHA
CSB-EP755945NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Histone-lysine N-methyltransferase set-9 (set-9), partial CSB-YP755946NHA
CSB-EP755946NHA
CSB-BP755946NHA
CSB-MP755946NHA
CSB-EP755946NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Peptidyl-prolyl isomerase cwc-27 (cwc-27), partial CSB-YP755947NHA
CSB-EP755947NHA
CSB-BP755947NHA
CSB-MP755947NHA
CSB-EP755947NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa GPI-anchored wall transfer protein 1 (gwt-1), partial CSB-YP755948NHA
CSB-EP755948NHA
CSB-BP755948NHA
CSB-MP755948NHA
CSB-EP755948NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa rRNA-processing protein cgr-1 (cgr-1) CSB-YP755949NHA
CSB-EP755949NHA
CSB-BP755949NHA
CSB-MP755949NHA
CSB-EP755949NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa PAB-dependent poly (A)-specific ribonuclease subunit pan-3 (pan-3), partial CSB-YP755950NHA
CSB-EP755950NHA
CSB-BP755950NHA
CSB-MP755950NHA
CSB-EP755950NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pre-mRNA-splicing factor slu-7 (slu-7) CSB-YP755951NHA
CSB-EP755951NHA
CSB-BP755951NHA
CSB-MP755951NHA
CSB-EP755951NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Eukaryotic translation initiation factor 3 subunit M (NCU02813) CSB-YP755952NHA
CSB-EP755952NHA
CSB-BP755952NHA
CSB-MP755952NHA
CSB-EP755952NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein cft-1 (cft-1), partial CSB-YP755953NHA
CSB-EP755953NHA
CSB-BP755953NHA
CSB-MP755953NHA
CSB-EP755953NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa ATP-dependent RNA helicase rok-1 (rok-1), partial CSB-YP755954NHA
CSB-EP755954NHA
CSB-BP755954NHA
CSB-MP755954NHA
CSB-EP755954NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Inosine-5'-monophosphate dehydrogenase (NCU03117), partial CSB-YP755955NHA
CSB-EP755955NHA
CSB-BP755955NHA
CSB-MP755955NHA
CSB-EP755955NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Cytosolic Fe-S cluster assembly factor nar-1 (nar-1), partial CSB-YP755956NHA
CSB-EP755956NHA
CSB-BP755956NHA
CSB-MP755956NHA
CSB-EP755956NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Vacuolar protein sorting/targeting protein 10 (vps10), partial CSB-YP755957NHA
CSB-EP755957NHA
CSB-BP755957NHA
CSB-MP755957NHA
CSB-EP755957NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa ATP-dependent (S)-NAD (P)H-hydrate dehydratase (NCU02513) CSB-YP755958NHA
CSB-EP755958NHA
CSB-BP755958NHA
CSB-MP755958NHA
CSB-EP755958NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa COP9 signalosome complex subunit 2 (csn-2) CSB-YP755959NHA
CSB-EP755959NHA
CSB-BP755959NHA
CSB-MP755959NHA
CSB-EP755959NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium berghei L-lactate dehydrogenase (PB000185.00.0) CSB-YP755960PLH
CSB-EP755960PLH
CSB-BP755960PLH
CSB-MP755960PLH
CSB-EP755960PLH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP755961TNT
CSB-EP755961TNT
CSB-BP755961TNT
CSB-MP755961TNT
CSB-EP755961TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobalt-containing nitrile hydratase subunit beta CSB-YP755962PHAR
CSB-EP755962PHAR
CSB-BP755962PHAR
CSB-MP755962PHAR
CSB-EP755962PHAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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