Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Protochlamydia amoebophila Probable phosphoketolase (pc0005), partial CSB-YP757345PAAD
CSB-EP757345PAAD
CSB-BP757345PAAD
CSB-MP757345PAAD
CSB-EP757345PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein phosphatase 1 regulatory subunit 11 (Ppp1r11) CSB-YP757346RA
CSB-EP757346RA
CSB-BP757346RA
CSB-MP757346RA
CSB-EP757346RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Valine--tRNA ligase, mitochondrial (Vars2), partial CSB-YP757347RA
CSB-EP757347RA
CSB-BP757347RA
CSB-MP757347RA
CSB-EP757347RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Large proline-rich protein BAG6 (Bag6), partial CSB-YP757348RA
CSB-EP757348RA
CSB-BP757348RA
CSB-MP757348RA
CSB-EP757348RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat G-protein-signaling modulator 3 (Gpsm3) CSB-YP757349RA
CSB-EP757349RA
CSB-BP757349RA
CSB-MP757349RA
CSB-EP757349RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP757350BFP
CSB-EP757350BFP
CSB-BP757350BFP
CSB-MP757350BFP
CSB-EP757350BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP757351BFP
CSB-EP757351BFP
CSB-BP757351BFP
CSB-MP757351BFP
CSB-EP757351BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP757352BFP
CSB-EP757352BFP
CSB-BP757352BFP
CSB-MP757352BFP
CSB-EP757352BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus UPF0337 protein Bd3330 (Bd3330) CSB-YP757353BFP
CSB-EP757353BFP
CSB-BP757353BFP
CSB-MP757353BFP
CSB-EP757353BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP757354BFP
CSB-EP757354BFP
CSB-BP757354BFP
CSB-MP757354BFP
CSB-EP757354BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 50S ribosomal protein L1 (rplA) CSB-YP757355BFP
CSB-EP757355BFP
CSB-BP757355BFP
CSB-MP757355BFP
CSB-EP757355BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 30S ribosomal protein S12 (rpsL) CSB-YP757356BFP
CSB-EP757356BFP
CSB-BP757356BFP
CSB-MP757356BFP
CSB-EP757356BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 30S ribosomal protein S3 (rpsC) CSB-YP757357BFP
CSB-EP757357BFP
CSB-BP757357BFP
CSB-MP757357BFP
CSB-EP757357BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 50S ribosomal protein L5 (rplE) CSB-YP757358BFP
CSB-EP757358BFP
CSB-BP757358BFP
CSB-MP757358BFP
CSB-EP757358BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 50S ribosomal protein L17 (rplQ) CSB-YP757359BFP
CSB-EP757359BFP
CSB-BP757359BFP
CSB-MP757359BFP
CSB-EP757359BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP757360BFP
CSB-EP757360BFP
CSB-BP757360BFP
CSB-MP757360BFP
CSB-EP757360BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP757361BFP
CSB-EP757361BFP
CSB-BP757361BFP
CSB-MP757361BFP
CSB-EP757361BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Isoleucine--tRNA ligase (ileS), partial CSB-YP757362BFP
CSB-EP757362BFP
CSB-BP757362BFP
CSB-MP757362BFP
CSB-EP757362BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Ribonuclease Y 2 (rny2), partial CSB-YP757363BFP
CSB-EP757363BFP
CSB-BP757363BFP
CSB-MP757363BFP
CSB-EP757363BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP757364BFP
CSB-EP757364BFP
CSB-BP757364BFP
CSB-MP757364BFP
CSB-EP757364BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>