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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis Apoptosis-inducing factor 2 (aifm2), partial CSB-YP756945XBE1
CSB-EP756945XBE1
CSB-BP756945XBE1
CSB-MP756945XBE1
CSB-EP756945XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serine/threonine-protein kinase Sgk1-B (sgk1-b) CSB-YP756946XBE
CSB-EP756946XBE
CSB-BP756946XBE
CSB-MP756946XBE
CSB-EP756946XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis OTU domain-containing protein 6B (otud6b) CSB-YP756947XBE
CSB-EP756947XBE
CSB-BP756947XBE
CSB-MP756947XBE
CSB-EP756947XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Phosphatidylinositide phosphatase SAC1 (sacm1l), partial CSB-YP756948XBE1
CSB-EP756948XBE1
CSB-BP756948XBE1
CSB-MP756948XBE1
CSB-EP756948XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Alanine aminotransferase 2 (gpt2), partial CSB-YP756949XBE
CSB-EP756949XBE
CSB-BP756949XBE
CSB-MP756949XBE
CSB-EP756949XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis WD repeat-containing protein 61 (wdr61) CSB-YP756950XBE
CSB-EP756950XBE
CSB-BP756950XBE
CSB-MP756950XBE
CSB-EP756950XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transcriptional adapter 1 (tada1) CSB-YP756951XBE
CSB-EP756951XBE
CSB-BP756951XBE
CSB-MP756951XBE
CSB-EP756951XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Serine protease HTRA1A (htra1a) CSB-YP756952DIL
CSB-EP756952DIL
CSB-BP756952DIL
CSB-MP756952DIL
CSB-EP756952DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Mannose-1-phosphate guanyltransferase alpha-A (gmppaa) CSB-YP756953DIL
CSB-EP756953DIL
CSB-BP756953DIL
CSB-MP756953DIL
CSB-EP756953DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative glycosyltransferase ALG1-like (ALG1L) CSB-YP756954HU
CSB-EP756954HU
CSB-BP756954HU
CSB-MP756954HU
CSB-EP756954HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nuclear receptor subfamily 2 group C member 1-A (nr2c1-a), partial CSB-YP756955XBE
CSB-EP756955XBE
CSB-BP756955XBE
CSB-MP756955XBE
CSB-EP756955XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Choline transporter-like protein 4 (slc44a4), partial CSB-YP756956XBE
CSB-EP756956XBE
CSB-BP756956XBE
CSB-MP756956XBE
CSB-EP756956XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis N (6)-adenine-specific DNA methyltransferase 2 (n6amt2) CSB-YP756957XBE
CSB-EP756957XBE
CSB-BP756957XBE
CSB-MP756957XBE
CSB-EP756957XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nucleoporin seh1-B (seh1l-b) CSB-YP756958XBE
CSB-EP756958XBE
CSB-BP756958XBE
CSB-MP756958XBE
CSB-EP756958XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein NDNF (ndnf), partial CSB-YP756959XBE
CSB-EP756959XBE
CSB-BP756959XBE
CSB-MP756959XBE
CSB-EP756959XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis G kinase-anchoring protein 1-A (gkap1-a) CSB-YP756960XBE
CSB-EP756960XBE
CSB-BP756960XBE
CSB-MP756960XBE
CSB-EP756960XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Golgin subfamily A member 5 (golga5), partial CSB-YP756961XBE
CSB-EP756961XBE
CSB-BP756961XBE
CSB-MP756961XBE
CSB-EP756961XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase mib1 (mib1), partial CSB-YP756962XBE
CSB-EP756962XBE
CSB-BP756962XBE
CSB-MP756962XBE
CSB-EP756962XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mediator of RNA polymerase II transcription subunit 25 (med25), partial CSB-YP756963XBE
CSB-EP756963XBE
CSB-BP756963XBE
CSB-MP756963XBE
CSB-EP756963XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Beclin-1 (becn1) CSB-YP756964XBE
CSB-EP756964XBE
CSB-BP756964XBE
CSB-MP756964XBE
CSB-EP756964XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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