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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP755682PIJ
CSB-EP755682PIJ
CSB-BP755682PIJ
CSB-MP755682PIJ
CSB-EP755682PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii DNA mismatch repair protein mutL (mutL), partial CSB-YP755683PIJ
CSB-EP755683PIJ
CSB-BP755683PIJ
CSB-MP755683PIJ
CSB-EP755683PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP755684PIJ
CSB-EP755684PIJ
CSB-BP755684PIJ
CSB-MP755684PIJ
CSB-EP755684PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S15 (rpsO) CSB-YP755685PIJ
CSB-EP755685PIJ
CSB-BP755685PIJ
CSB-MP755685PIJ
CSB-EP755685PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP755686PIJ
CSB-EP755686PIJ
CSB-BP755686PIJ
CSB-MP755686PIJ
CSB-EP755686PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP755687PIJ
CSB-EP755687PIJ
CSB-BP755687PIJ
CSB-MP755687PIJ
CSB-EP755687PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Pseudouridine-5'-phosphate glycosidase 2 (psuG2) CSB-YP755688PIJ
CSB-EP755688PIJ
CSB-BP755688PIJ
CSB-MP755688PIJ
CSB-EP755688PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Elongation factor P (efp) CSB-YP755689PIJ
CSB-EP755689PIJ
CSB-BP755689PIJ
CSB-MP755689PIJ
CSB-EP755689PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP755690PIJ
CSB-EP755690PIJ
CSB-BP755690PIJ
CSB-MP755690PIJ
CSB-EP755690PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP755691PIJ
CSB-EP755691PIJ
CSB-BP755691PIJ
CSB-MP755691PIJ
CSB-EP755691PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP755692PIJ
CSB-EP755692PIJ
CSB-BP755692PIJ
CSB-MP755692PIJ
CSB-EP755692PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Trigger factor (tig) CSB-YP755693PIJ
CSB-EP755693PIJ
CSB-BP755693PIJ
CSB-MP755693PIJ
CSB-EP755693PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Recombination protein RecR (recR) CSB-YP755694PIJ
CSB-EP755694PIJ
CSB-BP755694PIJ
CSB-MP755694PIJ
CSB-EP755694PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Biosynthetic arginine decarboxylase (speA), partial CSB-YP755695PIJ
CSB-EP755695PIJ
CSB-BP755695PIJ
CSB-MP755695PIJ
CSB-EP755695PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Protein MraZ (mraZ) CSB-YP755696PIJ
CSB-EP755696PIJ
CSB-BP755696PIJ
CSB-MP755696PIJ
CSB-EP755696PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP755697PIJ
CSB-EP755697PIJ
CSB-BP755697PIJ
CSB-MP755697PIJ
CSB-EP755697PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribose-5-phosphate isomerase A (rpiA) CSB-YP755698PIJ
CSB-EP755698PIJ
CSB-BP755698PIJ
CSB-MP755698PIJ
CSB-EP755698PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Probable multidrug resistance protein norM (norM), partial CSB-YP755699PIJ
CSB-EP755699PIJ
CSB-BP755699PIJ
CSB-MP755699PIJ
CSB-EP755699PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Chaperone protein hscA (hscA), partial CSB-YP755700PIJ
CSB-EP755700PIJ
CSB-BP755700PIJ
CSB-MP755700PIJ
CSB-EP755700PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Urocanate hydratase (hutU), partial CSB-YP755701PIJ
CSB-EP755701PIJ
CSB-BP755701PIJ
CSB-MP755701PIJ
CSB-EP755701PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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