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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bordetella pertussis Cytidylate kinase (cmk) CSB-YP756483BUA
CSB-EP756483BUA
CSB-BP756483BUA
CSB-MP756483BUA
CSB-EP756483BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP756484BUA1
CSB-EP756484BUA1
CSB-BP756484BUA1
CSB-MP756484BUA1
CSB-EP756484BUA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 50S ribosomal protein L21 (rplU) CSB-YP756485BUA
CSB-EP756485BUA
CSB-BP756485BUA
CSB-MP756485BUA
CSB-EP756485BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Aspartate carbamoyltransferase (pyrB) CSB-YP756486BUA
CSB-EP756486BUA
CSB-BP756486BUA
CSB-MP756486BUA
CSB-EP756486BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Fumarate hydratase class II (fumC) CSB-YP756487BUA
CSB-EP756487BUA
CSB-BP756487BUA
CSB-MP756487BUA
CSB-EP756487BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Glycine cleavage system H protein (gcvH) CSB-YP756488BUA
CSB-EP756488BUA
CSB-BP756488BUA
CSB-MP756488BUA
CSB-EP756488BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Lipoyl synthase (lipA) CSB-YP756489BUA
CSB-EP756489BUA
CSB-BP756489BUA
CSB-MP756489BUA
CSB-EP756489BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP756490BUA
CSB-EP756490BUA
CSB-BP756490BUA
CSB-MP756490BUA
CSB-EP756490BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP756491BIK
CSB-EP756491BIK
CSB-BP756491BIK
CSB-MP756491BIK
CSB-EP756491BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose-phosphate pyrophosphokinase (prs) CSB-YP756492BIK
CSB-EP756492BIK
CSB-BP756492BIK
CSB-MP756492BIK
CSB-EP756492BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP756493BIK
CSB-EP756493BIK
CSB-BP756493BIK
CSB-MP756493BIK
CSB-EP756493BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine hydroxymethyltransferase 1 (glyA1) CSB-YP756494BIK
CSB-EP756494BIK
CSB-BP756494BIK
CSB-MP756494BIK
CSB-EP756494BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carboxylate-amine ligase BPP0286 (BPP0286) CSB-YP756495BIK
CSB-EP756495BIK
CSB-BP756495BIK
CSB-MP756495BIK
CSB-EP756495BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP756496BIK
CSB-EP756496BIK
CSB-BP756496BIK
CSB-MP756496BIK
CSB-EP756496BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malate synthase G (glcB), partial CSB-YP756497BIK
CSB-EP756497BIK
CSB-BP756497BIK
CSB-MP756497BIK
CSB-EP756497BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S13 (rpsM) CSB-YP756498BIK
CSB-EP756498BIK
CSB-BP756498BIK
CSB-MP756498BIK
CSB-EP756498BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L24 (rplX) CSB-YP756499BIK
CSB-EP756499BIK
CSB-BP756499BIK
CSB-MP756499BIK
CSB-EP756499BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L2 (rplB) CSB-YP756500BIK
CSB-EP756500BIK
CSB-BP756500BIK
CSB-MP756500BIK
CSB-EP756500BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S12 (rpsL) CSB-YP756501BIK
CSB-EP756501BIK
CSB-BP756501BIK
CSB-MP756501BIK
CSB-EP756501BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP756502BIK
CSB-EP756502BIK
CSB-BP756502BIK
CSB-MP756502BIK
CSB-EP756502BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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