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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Haemophilus ducreyi tRNA pseudouridine synthase A (truA) CSB-YP756083HSY
CSB-EP756083HSY
CSB-BP756083HSY
CSB-MP756083HSY
CSB-EP756083HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP756084BHM
CSB-EP756084BHM
CSB-BP756084BHM
CSB-MP756084BHM
CSB-EP756084BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP756085BHM
CSB-EP756085BHM
CSB-BP756085BHM
CSB-MP756085BHM
CSB-EP756085BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP756086BIK
CSB-EP756086BIK
CSB-BP756086BIK
CSB-MP756086BIK
CSB-EP756086BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica CCA-adding enzyme (cca) CSB-YP756087BTY
CSB-EP756087BTY
CSB-BP756087BTY
CSB-MP756087BTY
CSB-EP756087BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cyanate hydratase (cynS) CSB-YP756088SSL
CSB-EP756088SSL
CSB-BP756088SSL
CSB-MP756088SSL
CSB-EP756088SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Aspartate--tRNA ligase (aspS), partial CSB-YP756089SSL
CSB-EP756089SSL
CSB-BP756089SSL
CSB-MP756089SSL
CSB-EP756089SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP756090SSL
CSB-EP756090SSL
CSB-BP756090SSL
CSB-MP756090SSL
CSB-EP756090SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP756091SSL
CSB-EP756091SSL
CSB-BP756091SSL
CSB-MP756091SSL
CSB-EP756091SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (SYNW2301) CSB-YP756092SSL
CSB-EP756092SSL
CSB-BP756092SSL
CSB-MP756092SSL
CSB-EP756092SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase chain 4 (ndhD), partial CSB-YP756093SSL
CSB-EP756093SSL
CSB-BP756093SSL
CSB-MP756093SSL
CSB-EP756093SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II 12 kDa extrinsic protein (psbU) CSB-YP756094SSL
CSB-EP756094SSL
CSB-BP756094SSL
CSB-MP756094SSL
CSB-EP756094SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Elongation factor Tu (tuf) CSB-YP756095SSL
CSB-EP756095SSL
CSB-BP756095SSL
CSB-MP756095SSL
CSB-EP756095SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L31 (rpmE) CSB-YP756096SSL
CSB-EP756096SSL
CSB-BP756096SSL
CSB-MP756096SSL
CSB-EP756096SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Adenylate kinase (adk) CSB-YP756097SSL
CSB-EP756097SSL
CSB-BP756097SSL
CSB-MP756097SSL
CSB-EP756097SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L14 (rplN) CSB-YP756098SSL
CSB-EP756098SSL
CSB-BP756098SSL
CSB-MP756098SSL
CSB-EP756098SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L3 (rplC) CSB-YP756099SSL
CSB-EP756099SSL
CSB-BP756099SSL
CSB-MP756099SSL
CSB-EP756099SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Bifunctional pantoate ligase/cytidylate kinase (panC/cmk), partial CSB-YP756100SSL
CSB-EP756100SSL
CSB-BP756100SSL
CSB-MP756100SSL
CSB-EP756100SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP756101SSL1
CSB-EP756101SSL1
CSB-BP756101SSL1
CSB-MP756101SSL1
CSB-EP756101SSL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glycine/sarcosine N-methyltransferase (bsmA) CSB-YP756102SSL
CSB-EP756102SSL
CSB-BP756102SSL
CSB-MP756102SSL
CSB-EP756102SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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