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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis 50S ribosomal protein L23P (rpl23p) CSB-YP757285MSB
CSB-EP757285MSB
CSB-BP757285MSB
CSB-MP757285MSB
CSB-EP757285MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Cobalt transport protein CbiN (cbiN), partial CSB-YP757286MSB1
CSB-EP757286MSB1
CSB-BP757286MSB1
CSB-MP757286MSB1
CSB-EP757286MSB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Preprotein translocase subunit SecE (secE), partial CSB-YP757287MSB
CSB-EP757287MSB
CSB-BP757287MSB
CSB-MP757287MSB
CSB-EP757287MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L18P (rpl18p) CSB-YP757288MSB
CSB-EP757288MSB
CSB-BP757288MSB
CSB-MP757288MSB
CSB-EP757288MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L24P (rpl24p) CSB-YP757289MSB
CSB-EP757289MSB
CSB-BP757289MSB
CSB-MP757289MSB
CSB-EP757289MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 5-formaminoimidazole-4-carboxamide-1- (beta)-D-ribofuranosyl 5'-monophosphate synthetase (purP) CSB-YP757290MSB
CSB-EP757290MSB
CSB-BP757290MSB
CSB-MP757290MSB
CSB-EP757290MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Chorismate synthase (aroC) CSB-YP757291MSB
CSB-EP757291MSB
CSB-BP757291MSB
CSB-MP757291MSB
CSB-EP757291MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L10e (rpl10e) CSB-YP757292MSB
CSB-EP757292MSB
CSB-BP757292MSB
CSB-MP757292MSB
CSB-EP757292MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable cobyric acid synthase (cobQ) CSB-YP757293MSB
CSB-EP757293MSB
CSB-BP757293MSB
CSB-MP757293MSB
CSB-EP757293MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable peroxiredoxin (MMP1174) CSB-YP757294MSB
CSB-EP757294MSB
CSB-BP757294MSB
CSB-MP757294MSB
CSB-EP757294MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0235 protein MMP1055 (MMP1055) CSB-YP757295MSB
CSB-EP757295MSB
CSB-BP757295MSB
CSB-MP757295MSB
CSB-EP757295MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamate--tRNA ligase (gltX), partial CSB-YP757296MSB
CSB-EP757296MSB
CSB-BP757296MSB
CSB-MP757296MSB
CSB-EP757296MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP757297MSB
CSB-EP757297MSB
CSB-BP757297MSB
CSB-MP757297MSB
CSB-EP757297MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UDP-N-acetylglucosamine 2-epimerase (wecB) CSB-YP757298MSB
CSB-EP757298MSB
CSB-BP757298MSB
CSB-MP757298MSB
CSB-EP757298MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Proline--tRNA ligase (proS) CSB-YP757299MSB
CSB-EP757299MSB
CSB-BP757299MSB
CSB-MP757299MSB
CSB-EP757299MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S2P (rps2p) CSB-YP757300MSB
CSB-EP757300MSB
CSB-BP757300MSB
CSB-MP757300MSB
CSB-EP757300MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable tRNA pseudouridine synthase D 2 (truD2) CSB-YP757301MSB
CSB-EP757301MSB
CSB-BP757301MSB
CSB-MP757301MSB
CSB-EP757301MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis O-phosphoseryl-tRNA (Sec) selenium transferase (spcS) CSB-YP757302MSB
CSB-EP757302MSB
CSB-BP757302MSB
CSB-MP757302MSB
CSB-EP757302MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0218 protein MMP0442 (MMP0442) CSB-YP757303MSB
CSB-EP757303MSB
CSB-BP757303MSB
CSB-MP757303MSB
CSB-EP757303MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Enolase (eno) CSB-YP757304MSB
CSB-EP757304MSB
CSB-BP757304MSB
CSB-MP757304MSB
CSB-EP757304MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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