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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Putative uncharacterized protein LOC100131404 CSB-YP757085HU
CSB-EP757085HU
CSB-BP757085HU
CSB-MP757085HU
CSB-EP757085HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nascent polypeptide-associated complex subunit alpha-like protein 3 (At5g13850) CSB-YP757086DOA
CSB-EP757086DOA
CSB-BP757086DOA
CSB-MP757086DOA
CSB-EP757086DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Root meristem growth factor 3 (rgf3) CSB-YP757087DOA
CSB-EP757087DOA
CSB-BP757087DOA
CSB-MP757087DOA
CSB-EP757087DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Inactive ubiquitin carboxyl-terminal hydrolase 54 (Usp54), partial CSB-YP757088RA
CSB-EP757088RA
CSB-BP757088RA
CSB-MP757088RA
CSB-EP757088RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serine protease inhibitor Kazal-type 14 (Spink14) CSB-YP757089RA
CSB-EP757089RA
CSB-BP757089RA
CSB-MP757089RA
CSB-EP757089RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat PAB-dependent poly (A)-specific ribonuclease subunit 2 (Pan2), partial CSB-YP757090RA
CSB-EP757090RA
CSB-BP757090RA
CSB-MP757090RA
CSB-EP757090RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Olfactory receptor 5M10 (OR5M10), partial CSB-YP757091HU1
CSB-EP757091HU1
CSB-BP757091HU1
CSB-MP757091HU1
CSB-EP757091HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Olfactory receptor 10K2 (OR10K2), partial CSB-YP757092HU1
CSB-EP757092HU1
CSB-BP757092HU1
CSB-MP757092HU1
CSB-EP757092HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Keratin, type I cytoskeletal 15 (Krt15) CSB-YP757093RA
CSB-EP757093RA
CSB-BP757093RA
CSB-MP757093RA
CSB-EP757093RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Keratin, type II cytoskeletal 1b (Krt77) CSB-YP757094MO
CSB-EP757094MO
CSB-BP757094MO
CSB-MP757094MO
CSB-EP757094MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ras-like protein family member 11A (Rasl11a) CSB-YP757095RA
CSB-EP757095RA
CSB-BP757095RA
CSB-MP757095RA
CSB-EP757095RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat E3 ubiquitin-protein ligase NHLRC1 (Nhlrc1) CSB-YP757096RA
CSB-EP757096RA
CSB-BP757096RA
CSB-MP757096RA
CSB-EP757096RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Aldose reductase A (alrA) CSB-YP757097DKK
CSB-EP757097DKK
CSB-BP757097DKK
CSB-MP757097DKK
CSB-EP757097DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein phosphatase 4 regulatory subunit 3A (SMEK1), partial CSB-YP757098HU
CSB-EP757098HU
CSB-BP757098HU
CSB-MP757098HU
CSB-EP757098HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tetratricopeptide repeat protein 21B (ttc21b), partial CSB-YP757099XBE
CSB-EP757099XBE
CSB-BP757099XBE
CSB-MP757099XBE
CSB-EP757099XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Toll-interacting protein B (tollip-b) CSB-YP757100XBE
CSB-EP757100XBE
CSB-BP757100XBE
CSB-MP757100XBE
CSB-EP757100XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DEP domain-containing protein 1A (depdc1a), partial CSB-YP757101XBE
CSB-EP757101XBE
CSB-BP757101XBE
CSB-MP757101XBE
CSB-EP757101XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis N-lysine methyltransferase setd6 (setd6) CSB-YP757102XBE
CSB-EP757102XBE
CSB-BP757102XBE
CSB-MP757102XBE
CSB-EP757102XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis BSD domain-containing protein 1-A (bsdc1-a) CSB-YP757103XBE
CSB-EP757103XBE
CSB-BP757103XBE
CSB-MP757103XBE
CSB-EP757103XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Insulin-like growth factor II-B (igf2-b) CSB-YP757104XBE
CSB-EP757104XBE
CSB-BP757104XBE
CSB-MP757104XBE
CSB-EP757104XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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