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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Ubiquitin carboxyl-terminal hydrolase 51 (USP51) CSB-YP758218HU
CSB-EP758218HU
CSB-BP758218HU
CSB-MP758218HU
CSB-EP758218HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ubiquitin carboxyl-terminal hydrolase 43 (USP43), partial CSB-YP758219HU
CSB-BP758219HU
CSB-MP758219HU
CSB-EP758219HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Transcriptional regulatory protein pmrA (pmrA) CSB-YP758220ESP
CSB-EP758220ESP
CSB-BP758220ESP
CSB-MP758220ESP
CSB-EP758220ESP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Secreted phosphoprotein 24 (spp2) CSB-YP758221OEI
CSB-EP758221OEI
CSB-BP758221OEI
CSB-MP758221OEI
CSB-EP758221OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein unc-13 homolog D (UNC13D), partial CSB-YP758222HU
CSB-EP758222HU
CSB-BP758222HU
CSB-MP758222HU
CSB-EP758222HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Beta-defensin 40 (Defb40) CSB-YP758223MO
CSB-EP758223MO
CSB-BP758223MO
CSB-MP758223MO
CSB-EP758223MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Putative glycosyltransferase ORF300 (ORF300) CSB-YP758224AFAE
CSB-EP758224AFAE
CSB-BP758224AFAE
CSB-MP758224AFAE
CSB-EP758224AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Putative transmembrane protein ORF807 (ORF807), partial CSB-YP758225AFAE
CSB-EP758225AFAE
CSB-BP758225AFAE
CSB-MP758225AFAE
CSB-EP758225AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF132 (ORF132) CSB-YP758226AFAE
CSB-EP758226AFAE
CSB-BP758226AFAE
CSB-MP758226AFAE
CSB-EP758226AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF65 (ORF65) CSB-YP758227AFAE
CSB-EP758227AFAE
CSB-BP758227AFAE
CSB-MP758227AFAE
CSB-EP758227AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF48 (ORF48) CSB-YP758228AFAE
CSB-EP758228AFAE
CSB-BP758228AFAE
CSB-MP758228AFAE
CSB-EP758228AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Putative zinc finger protein ORF59a (ORF59a) CSB-YP758229AFAE
CSB-EP758229AFAE
CSB-BP758229AFAE
CSB-MP758229AFAE
CSB-EP758229AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Linear gramicidin synthase subunit C (lgrC), partial CSB-YP758230BWM
CSB-EP758230BWM
CSB-BP758230BWM
CSB-MP758230BWM
CSB-EP758230BWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Peptidoglycan-recognition protein SB1 (PGRP-SB1) CSB-YP758231DLU
CSB-EP758231DLU
CSB-BP758231DLU
CSB-MP758231DLU
CSB-EP758231DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Platanista minor Cytochrome b (MT-CYB), partial CSB-YP758232PHAM
CSB-EP758232PHAM
CSB-BP758232PHAM
CSB-MP758232PHAM
CSB-EP758232PHAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eschrichtius gibbosus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP758233EHC1
CSB-EP758233EHC1
CSB-BP758233EHC1
CSB-MP758233EHC1
CSB-EP758233EHC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse von Willebrand factor A domain-containing protein 2 (Vwa2) CSB-YP758234MO
CSB-EP758234MO
CSB-BP758234MO
CSB-MP758234MO
CSB-EP758234MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thylamys elegans Cytochrome b (MT-CYB), partial CSB-YP758235TCAR
CSB-EP758235TCAR
CSB-BP758235TCAR
CSB-MP758235TCAR
CSB-EP758235TCAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhyncholestes raphanurus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP758236RCAE1
CSB-EP758236RCAE1
CSB-BP758236RCAE1
CSB-MP758236RCAE1
CSB-EP758236RCAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD (P)H-quinone oxidoreductase subunit H, chloroplastic (ndhH) CSB-YP758237AIQ
CSB-EP758237AIQ
CSB-BP758237AIQ
CSB-MP758237AIQ
CSB-EP758237AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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