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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio Nucleoporin NUP53 (nup35) CSB-YP757585DIL
CSB-EP757585DIL
CSB-BP757585DIL
CSB-MP757585DIL
CSB-EP757585DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Membrane magnesium transporter 2 (Mmgt2), partial CSB-YP757586RA
CSB-EP757586RA
CSB-BP757586RA
CSB-MP757586RA
CSB-EP757586RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat TATA box-binding protein-associated factor, RNA polymerase I, subunit C (Taf1c), partial CSB-YP757587RA
CSB-EP757587RA
CSB-BP757587RA
CSB-MP757587RA
CSB-EP757587RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Leucine-rich repeat-containing protein 26 (Lrrc26), partial CSB-YP757588RA
CSB-EP757588RA
CSB-BP757588RA
CSB-MP757588RA
CSB-EP757588RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Probable G-protein coupled receptor 146 (gpr146), partial CSB-YP757589XBE1
CSB-EP757589XBE1
CSB-BP757589XBE1
CSB-MP757589XBE1
CSB-EP757589XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis UPF0361 protein C3orf37 homolog (TGas027m07.1) CSB-YP757590XBF
CSB-EP757590XBF
CSB-BP757590XBF
CSB-MP757590XBF
CSB-EP757590XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat BRCA1-A complex subunit BRE (Bre) CSB-YP757591RA
CSB-EP757591RA
CSB-BP757591RA
CSB-MP757591RA
CSB-EP757591RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse SH3KBP1-binding protein 1 (Shkbp1) CSB-YP757592MO
CSB-EP757592MO
CSB-BP757592MO
CSB-MP757592MO
CSB-EP757592MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Mitochondrial import receptor subunit TOM40 homolog (tomm40) CSB-YP757593XBF
CSB-EP757593XBF
CSB-BP757593XBF
CSB-MP757593XBF
CSB-EP757593XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Microtubule-associated protein RP/EB family member 1 (mapre1) CSB-YP757594XBF
CSB-EP757594XBF
CSB-BP757594XBF
CSB-MP757594XBF
CSB-EP757594XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein PRRC1 (prrc1) CSB-YP757595XBF
CSB-EP757595XBF
CSB-BP757595XBF
CSB-MP757595XBF
CSB-EP757595XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Forkhead box protein I1-ema (foxi1e) CSB-YP757596XBF
CSB-EP757596XBF
CSB-BP757596XBF
CSB-MP757596XBF
CSB-EP757596XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis NEDD8-conjugating enzyme Ubc12 (ube2m) CSB-YP757597XBF
CSB-EP757597XBF
CSB-BP757597XBF
CSB-MP757597XBF
CSB-EP757597XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Creatine kinase S-type, mitochondrial (Ckmt2) CSB-BP757598MO
CSB-MP757598MO
CSB-EP757598MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse UPF0740 protein C1orf192 homolog CSB-YP757599MO
CSB-EP757599MO
CSB-BP757599MO
CSB-MP757599MO
CSB-EP757599MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Thioredoxin domain-containing protein 2 (Txndc2) CSB-YP757600MO
CSB-EP757600MO
CSB-BP757600MO
CSB-MP757600MO
CSB-EP757600MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM171B (FAM171B), partial CSB-YP757601HU
CSB-EP757601HU
CSB-BP757601HU
CSB-MP757601HU
CSB-EP757601HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Connector enhancer of kinase suppressor of ras 3 (CNKSR3) CSB-YP757602HU
CSB-EP757602HU
CSB-BP757602HU
CSB-MP757602HU
CSB-EP757602HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Kinesin-like protein KIF15 (Kif15), partial CSB-YP757603MO
CSB-EP757603MO
CSB-BP757603MO
CSB-MP757603MO
CSB-EP757603MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Nucleoside diphosphate-linked moiety X motif 22 (Nudt22) CSB-YP757604RA
CSB-EP757604RA
CSB-BP757604RA
CSB-MP757604RA
CSB-EP757604RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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