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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 4-hydroxy-2-oxovalerate aldolase (MAP_0533) CSB-YP758599MLA
CSB-EP758599MLA
CSB-BP758599MLA
CSB-MP758599MLA
CSB-EP758599MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type III pantothenate kinase (coaX) CSB-YP758600MLA
CSB-EP758600MLA
CSB-BP758600MLA
CSB-MP758600MLA
CSB-EP758600MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Recombination protein RecR (recR) CSB-YP758601MLA
CSB-EP758601MLA
CSB-BP758601MLA
CSB-MP758601MLA
CSB-EP758601MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prephenate dehydratase (pheA) CSB-YP758602MLA
CSB-EP758602MLA
CSB-BP758602MLA
CSB-MP758602MLA
CSB-EP758602MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP758603GBK
CSB-EP758603GBK
CSB-BP758603GBK
CSB-MP758603GBK
CSB-EP758603GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens NADH-quinone oxidoreductase subunit I 2 (nuoI2) CSB-YP758604GBK
CSB-EP758604GBK
CSB-BP758604GBK
CSB-MP758604GBK
CSB-EP758604GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Cysteine--tRNA ligase (cysS) CSB-YP758605GBK
CSB-EP758605GBK
CSB-BP758605GBK
CSB-MP758605GBK
CSB-EP758605GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UvrABC system protein C (uvrC), partial CSB-YP758606GBK
CSB-EP758606GBK
CSB-BP758606GBK
CSB-MP758606GBK
CSB-EP758606GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UPF0234 protein GSU3204 (GSU3204) CSB-YP758607GBK
CSB-EP758607GBK
CSB-BP758607GBK
CSB-MP758607GBK
CSB-EP758607GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Peptide chain release factor 1 (prfA) CSB-YP758608GBK
CSB-EP758608GBK
CSB-BP758608GBK
CSB-MP758608GBK
CSB-EP758608GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Cell division protein FtsQ (ftsQ), partial CSB-YP758609GBK
CSB-EP758609GBK
CSB-BP758609GBK
CSB-MP758609GBK
CSB-EP758609GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Cobalt transport protein CbiM (cbiM), partial CSB-YP758610GBK
CSB-EP758610GBK
CSB-BP758610GBK
CSB-MP758610GBK
CSB-EP758610GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L7/L12 (rplL) CSB-YP758611GBK
CSB-EP758611GBK
CSB-BP758611GBK
CSB-MP758611GBK
CSB-EP758611GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S3 (rpsC) CSB-YP758612GBK
CSB-EP758612GBK
CSB-BP758612GBK
CSB-MP758612GBK
CSB-EP758612GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 50S ribosomal protein L6 (rplF) CSB-YP758613GBK
CSB-EP758613GBK
CSB-BP758613GBK
CSB-MP758613GBK
CSB-EP758613GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens 30S ribosomal protein S11 (rpsK) CSB-YP758614GBK
CSB-EP758614GBK
CSB-BP758614GBK
CSB-MP758614GBK
CSB-EP758614GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens UPF0145 protein GSU2791 (GSU2791) CSB-YP758615GBK
CSB-EP758615GBK
CSB-BP758615GBK
CSB-MP758615GBK
CSB-EP758615GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP758616GBK
CSB-EP758616GBK
CSB-BP758616GBK
CSB-MP758616GBK
CSB-EP758616GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Probable potassium transport system protein kup 2 (kup2), partial CSB-YP758617GBK
CSB-EP758617GBK
CSB-BP758617GBK
CSB-MP758617GBK
CSB-EP758617GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Probable potassium transport system protein kup 1 (kup1), partial CSB-YP758618GBK
CSB-EP758618GBK
CSB-BP758618GBK
CSB-MP758618GBK
CSB-EP758618GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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