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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Cyclin-A3-1 (CYCA3-1) CSB-YP758799OFG
CSB-EP758799OFG
CSB-BP758799OFG
CSB-MP758799OFG
CSB-EP758799OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable 6-phosphogluconolactonase 1 (Os03g0416500, LOC_Os03g30300) CSB-YP758800OFG
CSB-EP758800OFG
CSB-BP758800OFG
CSB-MP758800OFG
CSB-EP758800OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum HssA/B-like protein 15 (hssl15) CSB-YP758801DKK
CSB-EP758801DKK
CSB-BP758801DKK
CSB-MP758801DKK
CSB-EP758801DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0271804 (DDB_G0271804) CSB-YP758802DKK
CSB-EP758802DKK
CSB-BP758802DKK
CSB-MP758802DKK
CSB-EP758802DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum ATP synthase subunit epsilon, mitochondrial (atp5e) CSB-YP758803DKK
CSB-EP758803DKK
CSB-BP758803DKK
CSB-MP758803DKK
CSB-EP758803DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative calcium up-regulated protein I (cupI), partial CSB-YP758804DKK
CSB-EP758804DKK
CSB-BP758804DKK
CSB-MP758804DKK
CSB-EP758804DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Isocitrate dehydrogenase [NADP] cytoplasmic (idhC) CSB-YP758805DKK
CSB-EP758805DKK
CSB-BP758805DKK
CSB-MP758805DKK
CSB-EP758805DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum 5'-nucleotidase domain-containing protein DDB_G0275467 (DDB_G0275467), partial CSB-YP758806DKK
CSB-EP758806DKK
CSB-BP758806DKK
CSB-MP758806DKK
CSB-EP758806DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cytochrome c biogenesis protein CCS1, chloroplastic (CCS1), partial CSB-YP758807OFG
CSB-EP758807OFG
CSB-BP758807OFG
CSB-MP758807OFG
CSB-EP758807OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable glucuronosyltransferase Os05g0123100 (Os05g0123100, LOC_Os05g03174), partial CSB-YP758808OFG
CSB-EP758808OFG
CSB-BP758808OFG
CSB-MP758808OFG
CSB-EP758808OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative FK506-binding protein 9-like protein (FKBP9L) CSB-YP758809HU
CSB-EP758809HU
CSB-BP758809HU
CSB-MP758809HU
CSB-EP758809HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein MEI2-like 3 (ML3), partial CSB-YP758810OFG
CSB-EP758810OFG
CSB-BP758810OFG
CSB-MP758810OFG
CSB-EP758810OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Fibrillin-3 (FBN3), partial CSB-YP758811HU
CSB-EP758811HU
CSB-BP758811HU
CSB-MP758811HU
CSB-EP758811HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio anguillarum Na (+)-translocating NADH-quinone reductase subunit B (nqrB), partial CSB-YP758812VEV
CSB-EP758812VEV
CSB-BP758812VEV
CSB-MP758812VEV
CSB-EP758812VEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gentiana triflora Peroxiredoxin Q, chloroplastic (AFP1) CSB-YP758813GBAB
CSB-EP758813GBAB
CSB-BP758813GBAB
CSB-MP758813GBAB
CSB-EP758813GBAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus kaustophilus Urease accessory protein UreE (ureE) CSB-YP758814GAAA
CSB-EP758814GAAA
CSB-BP758814GAAA
CSB-MP758814GAAA
CSB-EP758814GAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Anoctamin-5 (Ano5), partial CSB-YP758815MO
CSB-EP758815MO
CSB-BP758815MO
CSB-MP758815MO
CSB-EP758815MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Quercus palustris Maturase K (matK), partial CSB-YP758816QAAE
CSB-EP758816QAAE
CSB-BP758816QAAE
CSB-MP758816QAAE
CSB-EP758816QAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Neuraminidase (NA), partial CSB-YP758817IHV1
CSB-EP758817IHV1
CSB-BP758817IHV1
CSB-MP758817IHV1
CSB-EP758817IHV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viburnum furcatum Furcatin hydrolase CSB-YP758818VAAX
CSB-EP758818VAAX
CSB-BP758818VAAX
CSB-MP758818VAAX
CSB-EP758818VAAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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