Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bothrops jararacussu Snake venom serine protease homolog CSB-YP759693BUX
CSB-EP759693BUX
CSB-BP759693BUX
CSB-MP759693BUX
CSB-EP759693BUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Mitoferrin-2 (slc25a28), partial CSB-YP759694DIL1
CSB-EP759694DIL1
CSB-BP759694DIL1
CSB-MP759694DIL1
CSB-EP759694DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Casein kinase I isoform delta-A (csnk1da) CSB-YP759695DIL
CSB-EP759695DIL
CSB-BP759695DIL
CSB-MP759695DIL
CSB-EP759695DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus flaviceps flaviceps Beta-bungarotoxin B chain CSB-YP759696BXM
CSB-EP759696BXM
CSB-BP759696BXM
CSB-MP759696BXM
CSB-EP759696BXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio N-alpha-acetyltransferase 35, NatC auxiliary subunit (naa35), partial CSB-YP759697DIL
CSB-EP759697DIL
CSB-BP759697DIL
CSB-MP759697DIL
CSB-EP759697DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Ufm1-specific protease 2 (ufsp2) CSB-YP759698DIL
CSB-EP759698DIL
CSB-BP759698DIL
CSB-MP759698DIL
CSB-EP759698DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Lissencephaly-1 homolog A (pafah1b1a) CSB-YP759699DIL
CSB-EP759699DIL
CSB-BP759699DIL
CSB-MP759699DIL
CSB-EP759699DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Polycomb complex protein BMI-1-B (bmi1b) CSB-YP759700DIL
CSB-EP759700DIL
CSB-BP759700DIL
CSB-MP759700DIL
CSB-EP759700DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombina maxima Kininogen-1a CSB-YP759701BIA
CSB-EP759701BIA
CSB-BP759701BIA
CSB-MP759701BIA
CSB-EP759701BIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyprinus carpio 60S ribosomal protein L15 (rpl15) CSB-YP759702EQE
CSB-EP759702EQE
CSB-BP759702EQE
CSB-MP759702EQE
CSB-EP759702EQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rutilus rutilus Opsin-VA, partial CSB-YP759703RIB1
CSB-EP759703RIB1
CSB-BP759703RIB1
CSB-MP759703RIB1
CSB-EP759703RIB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Zygote arrest protein 1 (zar1) CSB-YP759704XBE
CSB-EP759704XBE
CSB-BP759704XBE
CSB-MP759704XBE
CSB-EP759704XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine coronavirus Non-structural protein 3b (3b), partial CSB-YP759705CHAH1
CSB-EP759705CHAH1
CSB-BP759705CHAH1
CSB-MP759705CHAH1
CSB-EP759705CHAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Probable capsid protein 2 (MIMI_R439), partial CSB-YP759706ADAZ
CSB-EP759706ADAZ
CSB-BP759706ADAZ
CSB-MP759706ADAZ
CSB-EP759706ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba polyphaga mimivirus Putative serine/threonine-protein kinase/receptor R831 (MIMI_R831), partial CSB-YP759707ADAZ
CSB-EP759707ADAZ
CSB-BP759707ADAZ
CSB-MP759707ADAZ
CSB-EP759707ADAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cestrum yellow leaf curling virus Uncharacterized protein 3 (ORF III) CSB-YP759708CHAI
CSB-EP759708CHAI
CSB-BP759708CHAI
CSB-MP759708CHAI
CSB-EP759708CHAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fiji disease virus Putative outer capsid protein p10 (S10), partial CSB-YP759709FAAM
CSB-EP759709FAAM
CSB-BP759709FAAM
CSB-MP759709FAAM
CSB-EP759709FAAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Monocarboxylate transporter 9 (Slc16a9), partial CSB-YP759710MO
CSB-EP759710MO
CSB-BP759710MO
CSB-MP759710MO
CSB-EP759710MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Beta-defensin 37 (Defb37) CSB-YP759711MO
CSB-EP759711MO
CSB-BP759711MO
CSB-MP759711MO
CSB-EP759711MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (Ndufa12) CSB-YP759712MO
CSB-EP759712MO
CSB-BP759712MO
CSB-MP759712MO
CSB-EP759712MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>