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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio vulnificus Imidazolonepropionase (hutI) CSB-YP759293VCQ
CSB-EP759293VCQ
CSB-BP759293VCQ
CSB-MP759293VCQ
CSB-EP759293VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus NAD-dependent protein deacylase (cobB) CSB-YP759294VCQ
CSB-EP759294VCQ
CSB-BP759294VCQ
CSB-MP759294VCQ
CSB-EP759294VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0234 protein VV1636 (VV1636) CSB-YP759295VCQ
CSB-EP759295VCQ
CSB-BP759295VCQ
CSB-MP759295VCQ
CSB-EP759295VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP759296VCQ
CSB-EP759296VCQ
CSB-BP759296VCQ
CSB-MP759296VCQ
CSB-EP759296VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus NAD-dependent malic enzyme 1 (maeA1), partial CSB-YP759297VCQ
CSB-EP759297VCQ
CSB-BP759297VCQ
CSB-MP759297VCQ
CSB-EP759297VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Trimethylamine-N-oxide reductase (torA), partial CSB-YP759298VCQ
CSB-EP759298VCQ
CSB-BP759298VCQ
CSB-MP759298VCQ
CSB-EP759298VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Histidine biosynthesis bifunctional protein HisB (hisB) CSB-YP759299VCQ
CSB-EP759299VCQ
CSB-BP759299VCQ
CSB-MP759299VCQ
CSB-EP759299VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 8-amino-7-oxononanoate synthase (bioF) CSB-YP759300VCQ
CSB-EP759300VCQ
CSB-BP759300VCQ
CSB-MP759300VCQ
CSB-EP759300VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Cytidylate kinase (cmk) CSB-YP759301VCQ
CSB-EP759301VCQ
CSB-BP759301VCQ
CSB-MP759301VCQ
CSB-EP759301VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Elongation factor P-like protein (VV1229) CSB-YP759302VCQ
CSB-EP759302VCQ
CSB-BP759302VCQ
CSB-MP759302VCQ
CSB-EP759302VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Tryptophan synthase alpha chain (trpA) CSB-YP759303VCQ
CSB-EP759303VCQ
CSB-BP759303VCQ
CSB-MP759303VCQ
CSB-EP759303VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Electron transport complex protein RnfG (rnfG), partial CSB-YP759304VCQ
CSB-EP759304VCQ
CSB-BP759304VCQ
CSB-MP759304VCQ
CSB-EP759304VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP759305VCQ
CSB-EP759305VCQ
CSB-BP759305VCQ
CSB-MP759305VCQ
CSB-EP759305VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Zinc import ATP-binding protein ZnuC (znuC) CSB-YP759306VCQ
CSB-EP759306VCQ
CSB-BP759306VCQ
CSB-MP759306VCQ
CSB-EP759306VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Flagellar P-ring protein (flgI) CSB-YP759307VCQ
CSB-EP759307VCQ
CSB-BP759307VCQ
CSB-MP759307VCQ
CSB-EP759307VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Outer-membrane lipoprotein LolB (lolB) CSB-YP759308VCQ
CSB-EP759308VCQ
CSB-BP759308VCQ
CSB-MP759308VCQ
CSB-EP759308VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP759309VCQ
CSB-EP759309VCQ
CSB-BP759309VCQ
CSB-MP759309VCQ
CSB-EP759309VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP759310VCQ
CSB-EP759310VCQ
CSB-BP759310VCQ
CSB-MP759310VCQ
CSB-EP759310VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Gamma-glutamyl phosphate reductase (proA) CSB-YP759311VCQ
CSB-EP759311VCQ
CSB-BP759311VCQ
CSB-MP759311VCQ
CSB-EP759311VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Chaperone protein DnaK (dnaK), partial CSB-YP759312VCQ
CSB-EP759312VCQ
CSB-BP759312VCQ
CSB-MP759312VCQ
CSB-EP759312VCQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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